Bovine preimplantation embryos exhibit dramatic biological changes between before and
after the 8-16-cell stage. Here we report a simple lipofection method to transfect siRNA into …

The RNA interference (RNAi) technology is a powerful tool for studies in functional
genomics. The aim of this study was to evaluate the effects of a cationic lipid-based small …

This study aimed to assess the efficiency and effects of insulin-like growth factor receptor-1
(IGF-IR) siRNA knockdown during bovine preimplantation embryonic development. In …

The aim of this study was to compare gene silencing in bovine zygotes when small
interfering RNAs (siRNAs) were introduced into bovine zygotes by microinjection or lipid …

Accumulating evidence indicates that paternally derived microRNAs play a crucial role in the
development of early embryos and are regarded as the key factor in the successful …

Abstract Development of tools that can manipulate gene expression specifically and
efficiently in the trophectoderm (TE) lineage would greatly aid understanding the roles of …

RNA interference (RNAi) technology using small interfering RNAs (siRNA) has been widely
used as a powerful tool to knock down gene expression in various organisms. In pig …

Binucleate trophoblast giant cells (BNC) characteristically appear early in gestation in the
bovine placenta. They secret pivotal hormones and cytokines for feto-maternal …

RNA interference is an important tool to study the gene function. Microinjection and
electroporation are usually used to transfer DNA, small interference RNA (siRNA) …

Gene expression analysis in preimplantation embryos has been used for answering
fundamental questions related to development, prediction of pregnancy outcome, and other …