Liquid storage of porcine in vitro-produced blastocysts; a practical approach for short storage

LM Haug, R Jochems, AH Gaustad, E Kommisrud… - Zygote, 2023 - cambridge.org
Commercial application of embryo transfer in pig breeding is dependent on the storage of
embryos. The aim of this study was to assess the embryo quality of in vitro-produced …

Porcine blastocyst viability and developmental potential is maintained for 48 h of liquid storage at 25 C without CO2 gassing

CA Martinez, JM Cambra, A Nohalez, I Parrilla… - Theriogenology, 2019 - Elsevier
Short-and medium-term storage of pig embryos has become relevant for commercial
application of non-surgical deep uterine embryo transfer (NsDU-ET) in the light of the strict …

Viability and developmental potential of porcine blastocysts preserved for short term in a chemically defined medium at ambient temperature

Q Lin, QA Le, K Takebayashi, M Hirata… - … in Domestic Animals, 2022 - Wiley Online Library
This study developed an efficient method for liquid storage of in vitro‐derived porcine
blastocysts at ambient temperature for 24 hr. We evaluated the effects of new chemically …

Development, DNA fragmentation and cell death in porcine embryos after 24 h storage under different conditions

FJR Pomar, DWB Ducro-Steverink, W Hazeleger… - Theriogenology, 2004 - Elsevier
For practical applications of porcine embryo transfer (ET) it is important to develop feasible
embryo storage conditions. The aim of the present study was to evaluate the effect of short …

Short‐term preservation of porcine zygotes at ambient temperature using a chemically defined medium

Q Lin, QA Le, K Takebayashi, M Hirata… - Animal Science …, 2022 - Wiley Online Library
We aimed to develop a simple method for the short‐term preservation of in vitro‐produced
porcine zygotes at 25° C for up to 2 days. Firstly, we evaluated the efficiency of three storage …

Simple storage (CO2-free) of porcine morulae for up to three days maintains the in vitro viability and developmental competence

CA Martinez, A Nohalez, I Parrilla, X Lucas… - Theriogenology, 2018 - Elsevier
The advancement of porcine embryo transfer (ET) technology is constrained by regulatory
hurdles (liquid nitrogen transportation) or, more importantly, the technical obstacles of using …

Cryotolerance of in vitro-produced porcine blastocysts is improved when using glucose instead of pyruvate and lactate during the first 2 days of embryo culture

M Castillo-Martín, M Yeste, R Morató… - Reproduction …, 2013 - CSIRO Publishing
The objective of the present study was to determine the effects of replacing glucose with
pyruvate and lactate during the first 48 h of in vitro culture (IVC) in NCSU-23 medium on …

The effect of temperature during liquid storage of in vitro–matured bovine oocytes on subsequent embryo development

T Suttirojpattana, T Somfai, S Matoba, T Nagai… - Theriogenology, 2016 - Elsevier
The aim of the present study was to optimize the temperature for the temporal storage of
matured bovine oocytes. In vitro–matured bovine oocytes were preserved in HEPES …

Cryotolerance of porcine in vitro-produced blastocysts relies on blastocyst stage and length of in vitro culture prior to vitrification

R Morató, M Castillo-Martín, M Yeste… - Reproduction, Fertility …, 2016 - CSIRO Publishing
The aim of our study was to assess whether the cryotolerance of in vitro-produced embryos
could be influenced by the length of in vitro culture and size of blastocoel cavity before …

Freezability of porcine blastocysts at different peri-hatching stages

H Nagashima, H Yamakawa, H Niemann - Theriogenology, 1992 - Elsevier
The freezability of porcine peri-hatching stage blastocysts was investigated by the
cryopreservation of embryos at− 196° C with 1.5 M glycerol and by thawing, followed by in …