High-intensity pulsed electromagnetic field-mediated gene electrotransfection in vitro

M Kranjc, J Dermol-Černe, T Potočnik… - International journal of …, 2022 - mdpi.com
A high-intensity pulsed electromagnetic field (HI-PEMF) is a non-invasive and non-contact
delivery method and may, as such, have an advantage over gene electrotransfer mediated …

[HTML][HTML] Contactless delivery of plasmid encoding EGFP in vivo by high-intensity pulsed electromagnetic field

M Kranjc, SK Brezar, G Serša, D Miklavčič - Bioelectrochemistry, 2021 - Elsevier
Abstract High-Intensity Pulsed Electromagnetic Fields (HI-PEMF) treatment is an emerging
noninvasive and contactless alternative to conventional electroporation, since the electric …

Electrotransfer of siRNA to silence enhanced green fluorescent protein in tumor mediated by a high intensity pulsed electromagnetic field

S Kranjc Brezar, M Kranjc, M Čemažar, S Buček… - Vaccines, 2020 - mdpi.com
The contactless high intensity pulsed electromagnetic field (HI-PEMF)-induced increase of
cell membrane permeability is similar to conventional electroporation, with the important …

Transfection by electroporation of cancer and primary cells using nanosecond and microsecond electric fields

E Radzevičiūtė, V Malyško-Ptašinskė, J Novickij… - Pharmaceutics, 2022 - mdpi.com
Gene transfer into primary immune cells as well as into cell lines is essential for scientific
and therapeutical applications. One of the methods used for gene transfer is electroporation …

Gene transfer by electroporation with high frequency bipolar pulses in vitro

T Potočnik, D Miklavčič, AM Lebar - Bioelectrochemistry, 2021 - Elsevier
High-frequency bipolar pulses (HF-BP) have been demonstrated to be efficient for
membrane permeabilization and irreversible electroporation. Since membrane …

Sub-microsecond electrotransfection using new modality of high frequency electroporation

V Novickij, A Balevičiūtė, P Ruzgys, S Šatkauskas… - …, 2020 - Elsevier
Micro-millisecond range electric field pulses have been used for decades to facilitate DNA
transfer into cells and tissues, while the growing number of clinical trials underline the strong …

Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond …

E Radzevičiūtė-Valčiukė, J Gečaitė… - … in Bioengineering and …, 2024 - frontiersin.org
Gene electrotransfer (GET) is non-viral gene delivery technique, also known as
electroporation-mediated gene delivery or electrotransfection. GET is a method used to …

Efficient gene transfection by electroporation—in vitro and in silico study of pulse parameters

T Potočnik, S Sachdev, T Polajžer, A Maček Lebar… - Applied Sciences, 2022 - mdpi.com
Gene electrotransfer (GET) is a widely used method for nucleic acids' delivery into cells. We
explored, evaluated, and demonstrated the potential use of different pulse durations for …

In vitro and in vivo electric field-mediated permeabilization, gene transfer, and expression

M Golzio, MP Rols, J Teissie - Methods, 2004 - Elsevier
Electropulsation is one of the non-viral methods successfully used to transfer genes into
living cells in vitro as in vivo. This approach shows promise in the field of gene and cellular …

Pulsed Electromagnetic Field Assisted in vitro Electroporation: A Pilot Study

V Novickij, A Grainys, E Lastauskienė… - Scientific reports, 2016 - nature.com
Electroporation is a phenomenon occurring due to exposure of cells to Pulsed Electric Fields
(PEF) which leads to increase of membrane permeability. Electroporation is used in …