Cointegrate-resolution of toluene-catabolic transposon Tn4651: determination of crossover site and the segment required for full resolution activity

H Yano, H Genka, Y Ohtsubo, Y Nagata, EM Top… - Plasmid, 2013 - Elsevier
Tn3-family transposon Tn4651 from Pseudomonas putida mt-2 plasmid pWW0 carries two
divergently transcribed genes, tnpS and tnpT, for cointegrate-resolution. While tnpS encodes …

Site-Specific Recombination System Encoded by Toluene Catabolic Transposon Tn4651

H Genka, Y Nagata, M Tsuda - Journal of bacteriology, 2002 - Am Soc Microbiol
The 56-kb class II toluene catabolic transposon Tn 4651 from Pseudomonas putida plasmid
pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution …

Toluene transposons Tn4651 and Tn4653 are class II transposons

M Tsuda, K Minegishi, T Iino - Journal of bacteriology, 1989 - Am Soc Microbiol
The toluene degradative transposon Tn4651 is included within another transposon, Tn4653,
and both of these elements are members of the Tn3 family. The tnpA gene product of each …

Genetic analysis of a transposon carrying toluene degrading genes on a TOL plasmid pWWO

M Tsuda, T Iino - Molecular and General Genetics MGG, 1987 - Springer
Toluene degrading (xyl) genes on a Pseudomonas TOL plasmid pWWO are located within a
39-kb DNA portion. The 56-kb region including these xyl genes and its 17-kb derivative with …

Identification and Characterization of Tn4656, a Novel Class II Transposon Carrying a Set of Toluene-Degrading Genes from TOL Plasmid pWW53

M Tsuda, H Genka - Journal of bacteriology, 2001 - Am Soc Microbiol
It has been reported that the toluene-degrading (xyl) genes from Pseudomonas putida
plasmid pWW53 are able to translocate to broad-host-range drug resistance plasmid RP4 …

Expression of the Transposase Gene tnpAof Tn4652 Is Positively Affected by Integration Host Factor

R Hõrak, M Kivisaar - Journal of bacteriology, 1998 - Am Soc Microbiol
Tn 4652 is a derivative of the toluene degradation transposon Tn 4651 that belongs to the
Tn 3 family of transposons (M. Tsuda and T. Iino, Mol. Gen. Genet. 210: 270–276, 1987). We …

Tn5 Transposase with an Altered Specificity for Transposon Ends

TA Naumann, WS Reznikoff - Journal of bacteriology, 2002 - Am Soc Microbiol
Tn 5 is a composite bacterial transposon that encodes a protein, transposase (Tnp), required
for movement of the transposon. The initial step in the transposition pathway involves …

Tn5 transposase loops DNA in the absence of Tn5 transposon end sequences

CD Adams, B Schnurr, D Skoko, JF Marko… - Molecular …, 2006 - Wiley Online Library
Transposases mediate transposition first by binding specific DNA end sequences that define
a transposable element and then by organizing protein and DNA into a highly structured and …

Enhancement and rescue of target capture in Tn10 transposition by site‐specific modifications in target DNA

PA Pribil, SJ Wardle, DB Haniford - Molecular microbiology, 2004 - Wiley Online Library
The bacterial transposon Tn10 inserts preferentially into specific target sequences. This
insertion specificity appears to be linked to the ability of target sites to adopt symmetrically …

Identification and characterization of Tn4653, a transposon covering the toluene transposon Tn4651 on TOL plasmid pWW0

M Tsuda, T Iino - Molecular and General Genetics MGG, 1988 - Springer
Summary A Pseudomonas TOL plasmid pWW0 possesses toluene degradative pathway
(xyl) genes. Unstable maintenance of a pWW0 derivative in Escherichia coli allowed us to …