Both the ability to freeze human spermatozoa and the possibility of pregnancy following
intrauterine insemination have existed for> 40 years. There have been a number of …

In the 1960s, sperm cryopreservation was developed as a method to preserve fertility.
Currently, techniques for the cryopreservation of human spermatozoa have been widely …

Three experiments were conducted to evaluate effects of (1) holding semen at+ 5.0° C for 30
minutes or− 5.0° C for 10 minutes and (2) ice crystal induction (seeding) on frozen-thawed …

New research on the cooling and cryopreservation of mammalian spermatozoa is reviewed
in the context of the older literature. Cryoinjury to a variety of cell organelles is regarded as …

OBJECTIVE: To evaluate the ability of two cryopreservation methods and three
cryoprotectants to preserve sperm quality. DESIGN: A prospective clinical study. SETTING …

A procedure is described that allows cryopreservation and efficient post-thaw recovery of
either a single or a small group of human spermatozoa. This is achieved by injecting them …

Postthaw dynamics of motility maintenance and ability to penetrate zona-free hamster ova
were examined with human sperm. Ten semen samples were each divided into two equal …

Freezing of human sperm is considered a routine procedure in assisted reproductive
technology (ART) laboratories. This article considers various aspects of cryopreservation of …

Human spermatozoa was relatively resistant to cooling shock. However, when diluted
semen was cooled faster than 10° C per minute from room temperature (RT) to 5° C and …

Human spermatozoa have unusual cryobiological behaviour and improvements in their
survival have not been achieved by the standard approaches of cryobiology. Conventional …