PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings
S Yang, RE Rothman - The Lancet infectious diseases, 2004 - thelancet.com
Molecular diagnostics are revolutionising the clinical practice of infectious disease. Their
effects will be significant in acute-care settings where timely and accurate diagnostic tools …
effects will be significant in acute-care settings where timely and accurate diagnostic tools …
Forty years of molecular diagnostics for infectious diseases
JE Schmitz, CW Stratton, DH Persing… - Journal of Clinical …, 2022 - Am Soc Microbiol
Nearly 40 years have elapsed since the invention of the PCR, with its extremely sensitive
and specific ability to detect nucleic acids via in vitro enzyme-mediated amplification. In turn …
and specific ability to detect nucleic acids via in vitro enzyme-mediated amplification. In turn …
[HTML][HTML] Real-time PCR in the microbiology laboratory
IM Mackay - Clinical microbiology and infection, 2004 - Elsevier
Use of PCR in the field of molecular diagnostics has increased to the point where it is now
accepted as the standard method for detecting nucleic acids from a number of sample and …
accepted as the standard method for detecting nucleic acids from a number of sample and …
Ten years of R&D and full automation in molecular diagnosis
A 10-year experience of our automated molecular diagnostic platform that carries out 91
different real-time PCR is described. Progresses and future perspectives in molecular …
different real-time PCR is described. Progresses and future perspectives in molecular …
Digital PCR applications for the diagnosis and management of infection in critical care medicine
I Merino, A de la Fuente, M Domínguez-Gil, JM Eiros… - Critical Care, 2022 - Springer
Infection (either community acquired or nosocomial) is a major cause of morbidity and
mortality in critical care medicine. Sepsis is present in up to 30% of all ICU patients. A large …
mortality in critical care medicine. Sepsis is present in up to 30% of all ICU patients. A large …
Quantitative real-time PCR is not more sensitive than “conventional” PCR
P Bastien, GW Procop, U Reischl - Journal of clinical microbiology, 2008 - Am Soc Microbiol
Molecular methods, essentially based upon PCR, have become an indispensable tool in the
diagnosis of infectious diseases. Real-time quantitative PCR (qrtPCR), as a novel …
diagnosis of infectious diseases. Real-time quantitative PCR (qrtPCR), as a novel …
Real-time PCR in clinical microbiology: applications for routine laboratory testing
MJ Espy, JR Uhl, LM Sloan… - Clinical microbiology …, 2006 - Am Soc Microbiol
Real-time PCR has revolutionized the way clinical microbiology laboratories diagnose many
human microbial infections. This testing method combines PCR chemistry with fluorescent …
human microbial infections. This testing method combines PCR chemistry with fluorescent …
Advances in microfluidic PCR for point-of-care infectious disease diagnostics
Global burdens from existing or emerging infectious diseases emphasize the need for point-
of-care (POC) diagnostics to enhance timely recognition and intervention. Molecular …
of-care (POC) diagnostics to enhance timely recognition and intervention. Molecular …
Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens
Q Liu, X Jin, J Cheng, H Zhou… - Molecular Medicine …, 2023 - spandidos-publications.com
Infectious diseases are a major global cause of morbidity and mortality, seriously affecting
public health and socioeconomic stability. Since infectious diseases can be caused by a …
public health and socioeconomic stability. Since infectious diseases can be caused by a …
Multiplex PCR: optimization and application in diagnostic virology
EM Elnifro, AM Ashshi, RJ Cooper… - Clinical microbiology …, 2000 - Am Soc Microbiol
SUMMARY PCR has revolutionized the field of infectious disease diagnosis. To overcome
the inherent disadvantage of cost and to improve the diagnostic capacity of the test …
the inherent disadvantage of cost and to improve the diagnostic capacity of the test …
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