Monitoring the proteins and lipids that mediate all cellular processes requires imaging
methods with increased spatial and temporal resolution. STED (stimulated emission …

Stimulated emission depletion (STED) nanoscopy allows observations of subcellular
dynamics at the nanoscale. Applications have, however, been severely limited by the lack of …

Stimulated emission depletion (STED) nanoscopy enables the visualization of subcellular
organelles in unprecedented detail. However, reducing the power dependency remains one …

Applying pulsed excitation together with time-gated detection improves the fluorescence on-
off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus …

Despite its short history, diffraction-unlimited fluorescence microscopy techniques have
already made a substantial imprint in the biological sciences. In this review, we describe …

Live-cell imaging has become indispensable in modern cell biology. However, many cellular
processes can only be resolved by the means of super-resolution microscopy. Among the …

Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while
preserving useful aspects of fluorescence microscopy, such as optical sectioning, and …

The well-known saying of “Seeing is believing” became even more apt in biology when
stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel …

Stimulation emission depletion (STED) microscopy breaks the spatial resolution limit of
conventional light microscopy while retaining its major advantages, such as working under …

Stimulated emission depletion (STED) microscopy was the first fluorescence microscopy
technique to break the classic diffraction barrier of light microscopy. Even though STED was …