Microbial community analysis via marker gene amplicon sequencing has become a routine
method in the field of soil research. In this perspective, we discuss technical challenges and …

The use of cultivation independent methods has revolutionized soil biology in the last
decades. Most popular approaches are based on directly extracted DNA from soil and …

It is thought that a microbial community is an assemblage of organisms, genes, and gene
functions. Transient, acute signals such as excessive nutrient loads or disturbance and …

Recent advances in molecular approaches and DNA sequencing have greatly progressed
the field of ecology and allowed for the study of complex communities in unprecedented …

Direct extraction and characterization of microbial community DNA through PCR amplicon
surveys and metagenomics has revolutionized the study of environmental microbiology and …

Next generation fungal amplicon sequencing is being used with increasing frequency to
study fungal diversity in various ecosystems; however, the influence of sample preparation …

Microorganisms are vital to the overall ecosystem functioning, stability, and sustainability.
Soil fertility and health depend on chemical composition and also on the qualitative and …

Nuclear large subunit ribosomal DNA is widely used in fungal phylogenetics and to an
increasing extent also amplicon-based environmental sequencing. The relatively short …

Fungi are important in soils as both decomposers and plant symbionts, and an
understanding of the composition of their complex communities is thus indispensable to …

Soil microbial community data produced by next-generation sequencing platforms has
introduced a new era in microbial ecology studies but poses a challenge for data analysis …