Towards construction of a novel nm resolution MeV-STEM for imaging of thick biological samples

X Yang, L Wang, J Maxson, A Bartnik… - arXiv preprint arXiv …, 2023 - arxiv.org
Driven by life-science applications, mega-electron-volt Scanning Transmission Electron
Microscope (MeV-STEM) has been proposed to image thick biological samples. The high …

Towards Construction of a Novel Nanometer-Resolution MeV-STEM for Imaging Thick Frozen Biological Samples

X Yang, L Wang, J Maxson, AC Bartnik, M Kaemingk… - Photonics, 2024 - mdpi.com
Driven by life-science applications, a mega-electron-volt Scanning Transmission Electron
Microscope (MeV-STEM) has been proposed here to image thick frozen biological samples …

Monte Carlo simulation of electron interactions in an MeV-stem for thick frozen biological sample imaging

L Wang, X Yang - Biophysical Journal, 2024 - cell.com
To visualize thick biological samples a variety of volume electron microscopy techniques
have been developed. In Array Tomography, sections prepared using microtomes, cryo …

Optimize Electron Beam Energy toward In Situ Imaging of Thick Frozen Bio-Samples with Nanometer Resolution Using MeV-STEM

X Yang, L Wang, V Smaluk, T Shaftan - Nanomaterials, 2024 - mdpi.com
To optimize electron energy for in situ imaging of large biological samples up to 10 μm in
thickness with nanoscale resolutions, we implemented an analytical model based on elastic …

Transmission imaging of biological tissue with the Delft multi‐beam SEM

W Zuidema, Y Ren, J Hoogenboom… - European …, 2016 - Wiley Online Library
A major bottleneck for large‐scale and volume EM is the imaging speed. The total
acquisition time needed for a single sample can easily take days, or even weeks using …

DELTA-A Novel Ultra-Low Voltage SEM for Electron Spectroscopic Imaging

RR Schroder, B Schindler, M Schnell… - Microscopy and …, 2018 - cambridge.org
In the last few years, low-voltage TEM [1] was instrumental for changing paradigms of
imaging using low energy electrons. For very thin and highly conductive samples low …

High-resolution low-dose scanning transmission electron microscopy

JP Buban, Q Ramasse, B Gipson… - Journal of electron …, 2010 - academic.oup.com
During the past two decades instrumentation in scanning transmission electron microscopy
(STEM) has pushed toward higher intensity electron probes to increase the signal-to-noise …

A dedicated multi-beam SEM for transmission imaging of thin samples

P Kruit, W Zuidema - Microscopy and Microanalysis, 2019 - cambridge.org
In a few successive PhD projects, we transformed a FEI Nova-nano SEM column into an
instrument with an array of 14x14 focused electron beams on the sample [1-3]. The original …

Low-dose imaging techniques for transmission electron microscopy

DB Carlson, JE Evans, K Maaz - The transmission electron …, 2012 - books.google.com
Transmission electron microscopes have the ability to visualize almost any specimen at the
nanoscale. Unfortunately, modified imaging protocols are needed when samples are …

Towards the low-dose characterization of beam sensitive nanostructures via implementation of sparse image acquisition in scanning transmission electron microscopy

S Hwang, CW Han, SV Venkatakrishnan… - Measurement …, 2017 - iopscience.iop.org
Scanning transmission electron microscopy (STEM) has been successfully utilized to
investigate atomic structure and chemistry of materials with atomic resolution. However …