Despite the surge of microbial genome data, experimental testing is important to confirm
inferences about the cell biology, ecological roles and evolution of microorganisms. As the …

The cultivation of bacteria is highly biased toward a few phylogenetic groups. Many of the
currently underexplored bacterial lineages likely have novel biosynthetic pathways and …

Any talk of the demise of in vitro cultivation as a useful mechanism for revealing many of
nature's past and present secrets appears to be unfounded and premature. The first years of …

The paradigm that only 1% of microbes are culturable has had a profound impact on our
understanding of microbial ecology and is still a major motivation for mostly using molecular …

Microorganisms (both pro‑and eukaryotes) occur in all marine environments with an
estimated abundance of 103–1010 cells/cm3 in sediments and 104–107 cells/ml in …

In terms of the number and diversity of living units, the prokaryotic empire is the most
represented form of life on Earth, and yet it is still to a significant degree shrouded in …

Compared to bacteria, our knowledge of archaeal biology is limited. Historically,
microbiologists have mostly relied on culturing and single-gene diversity surveys to …

Archaea are a domain of prokaryotic organisms with intriguing physiological characteristics
and ecological importance. In Microbial Biotechnology, archaea are historically …

A huge number of microorganisms are still unknown and uncultivated. SSU rRNA gene-
based analyses of complex microbial communities are unveiling their diversity, distribution …

Since the invention of the Petri dish, there have been continuous efforts to improve efficiency
in microbial cultivation. These efforts were devoted to the attainment for diverse growth …