Separation of glycoproteins based on sugar chains using novel stationary phases modified with poly (ethylene glycol)-conjugated boronic-acid derivatives

H Kobayashi, Y Masuda, H Takaya, T Kubo… - Analytical …, 2022 - ACS Publications
Boronic acid (BA) reversibly complexes with the diol structure. BA derivatives separate
glycoproteins based on the differences in the sugar chains. Separation typically occurs …

Novel boronate material affords efficient enrichment of glycopeptides by synergized hydrophilic and affinity interactions

J Chen, X Li, M Feng, K Luo, J Yang… - Analytical and bioanalytical …, 2017 - Springer
Abstract Development of novel materials for enrichment of glycopeptides is the key to a
comprehensive analysis of the glycoproteome, which is closely related to several major …

A high boronate avidity monolithic capillary for the selective enrichment of trace glycoproteins

D Li, Y Li, X Li, Z Bie, X Pan, Q Zhang, Z Liu - Journal of Chromatography A, 2015 - Elsevier
Boronate affinity materials, as effective sample enrichment sorbents for glycoproteomic
analysis, have attracted increasing attention in recent years. However, most of boronate …

Preparation and characterization of fluorophenylboronic acid-functionalized monolithic columns for high affinity capture of cis-diol containing compounds

Q Li, C Lü, Z Liu - Journal of Chromatography A, 2013 - Elsevier
Boronic acids are important ligands for the selective recognition and capture of cis-diol
containing compounds, such as nucleosides and glycoproteins. In a recent study, it was …

Off-line hyphenation of boronate affinity monolith-based extraction with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for efficient analysis …

Z Bie, Y Chen, H Li, R Wu, Z Liu - Analytica chimica acta, 2014 - Elsevier
Boronate affinity materials have attracted increasing attentions as sample enrichment
platforms for glycoproteomic analysis in recent years. However, most of the boronate affinity …

“Clickable” affinity ligands for effective separation of glycoproteins

T Suksrichavalit, K Yoshimatsu… - … of Chromatography A, 2010 - Elsevier
In this paper, we present a new modular approach to immobilize boronic acid ligands that
can offer effective separation of glycoproteins. A new “clickable” boronic acid ligand was …

Back to BAC: insights into boronate affinity chromatography interaction mechanisms

MB Espina-Benitez, J Randon… - Separation & …, 2018 - Taylor & Francis
Boronate affinity chromatography (BAC) is commonly used in sample preparation
techniques for its specific selectivity for cis-diol-containing compounds. Various …

Branched polyethyleneimine-assisted boronic acid-functionalized silica nanoparticles for the selective enrichment of trace glycoproteins

D Li, H Xia, L Wang - Talanta, 2018 - Elsevier
Boronate affinity materials have attracted more and more attention in extraction, separation
and enrichment of glycoproteins due to the important roles that glycoproteins take on in …

A benzoboroxole‐based affinity ligand for glycoprotein purification at physiological pH

L Rowe, G El Khoury, CR Lowe - Journal of Molecular …, 2016 - Wiley Online Library
Developing ligands capable of carbohydrate recognition has become increasingly important
as the essential roles of glycoproteins and glycolipids in a diverse array of cellular signaling …

Shielding of protein–boronate interactions during boronate chromatography of neoglycoproteins

YC Li, EL Larsson, H Jungvid, IY Galaev… - … of Chromatography A, 2001 - Elsevier
A method for separating glycoproteins on a boronate column under conditions which
suppress the interactions between the protein moiety and the boronic acid ligand has been …