[HTML][HTML] Single-cell RNA-seq technologies and related computational data analysis

G Chen, B Ning, T Shi - Frontiers in genetics, 2019 - frontiersin.org
Single-cell RNA sequencing (scRNA-seq) technologies allow the dissection of gene
expression at single-cell resolution, which greatly revolutionizes transcriptomic studies. A …

Introduction to single‐cell RNA sequencing

TK Olsen, N Baryawno - Current protocols in molecular biology, 2018 - Wiley Online Library
During the last decade, high‐throughput sequencing methods have revolutionized the entire
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …

Single-cell RNA sequencing analysis: a step-by-step overview

S Slovin, A Carissimo, F Panariello, A Grimaldi… - RNA …, 2021 - Springer
Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …

Granatum: a graphical single-cell RNA-Seq analysis pipeline for genomics scientists

X Zhu, TK Wolfgruber, A Tasato, C Arisdakessian… - Genome medicine, 2017 - Springer
Background Single-cell RNA sequencing (scRNA-Seq) is an increasingly popular platform
to study heterogeneity at the single-cell level. Computational methods to process scRNA …

ASAP: a web-based platform for the analysis and interactive visualization of single-cell RNA-seq data

V Gardeux, FPA David, A Shajkofci, PC Schwalie… - …, 2017 - academic.oup.com
Motivation Single-cell RNA-sequencing (scRNA-seq) allows whole transcriptome profiling of
thousands of individual cells, enabling the molecular exploration of tissues at the cellular …

Exploring the single-cell RNA-seq analysis landscape with the scRNA-tools database

L Zappia, B Phipson, A Oshlack - PLoS computational biology, 2018 - journals.plos.org
As single-cell RNA-sequencing (scRNA-seq) datasets have become more widespread the
number of tools designed to analyse these data has dramatically increased. Navigating the …

scRNASeqDB: a database for RNA-Seq based gene expression profiles in human single cells

Y Cao, J Zhu, P Jia, Z Zhao - Genes, 2017 - mdpi.com
Single-cell RNA sequencing (scRNA-Seq) is rapidly becoming a powerful tool for high-
throughput transcriptomic analysis of cell states and dynamics at the single cell level. Both …

Over 1000 tools reveal trends in the single-cell RNA-seq analysis landscape

L Zappia, FJ Theis - Genome biology, 2021 - Springer
Recent years have seen a revolution in single-cell RNA-sequencing (scRNA-seq)
technologies, datasets, and analysis methods. Since 2016, the scRNA-tools database has …

A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications

A Haque, J Engel, SA Teichmann, T Lönnberg - Genome medicine, 2017 - Springer
RNA sequencing (RNA-seq) is a genomic approach for the detection and quantitative
analysis of messenger RNA molecules in a biological sample and is useful for studying …

Goals and approaches for each processing step for single-cell RNA sequencing data

Z Zhang, F Cui, C Wang, L Zhao… - Briefings in …, 2021 - academic.oup.com
Single-cell RNA sequencing (scRNA-seq) has enabled researchers to study gene
expression at the cellular level. However, due to the extremely low levels of transcripts in a …