Since the first reported transfection studies using cationic liposomes in 1987, significant
advances have been made on the understanding of the physical properties of DNA/cationic …

Cationic lipid/DNA complexes (lipoplexes) are promising vehicles for DNA vaccines or gene
therapy. In these systems, transfection efficiency is highly related to lipoplex charge ratio …

Formation of liposome/polynucleotide complexes (lipoplexes) involves electrostatic
interactions, which induce changes in liposome structure. The ability of these complexes to …

Fluorescence resonance energy transfer (FRET) was used to monitor interactions between
Cy3-labeled plasmid DNA and NBD-labeled cationic liposomes. FRET data show that …

Gene-based therapeutic approaches are based upon the concept that, if a disease is
caused by a mutation in a gene, then adding back the wild-type gene should restore regular …

Publisher Summary This chapter describes some experimental procedures of biophysical
characterization of cationic liposome–DNA complexes and their interaction with cells. The …

Lipid–DNA complexes (lipoplexes) are widely used, since several years, as gene carriers.
However, their transfection efficiency, both in vitro and in vivo, depends, in a rather complex …

A flow fluorometric analysis (using a commercial flow cytometer) of individual cationic lipoid–
DNA complexes is presented. Such single lipoplex studies have the advantage of providing …

Lipoplexes, which are formed spontaneously between cationic liposomes and negatively
charged nucleic acids, are commonly used for gene and oligonucleotide delivery in vitro and …

DNA-binding cationic liposomes are routinely employed as one of the major non-viral
transfecting agents delivering DNA and other genes inside the cells. Cationic liposomes …