Genetically encoded ATP biosensors for direct monitoring of cellular ATP dynamics

D White III, Q Yang - Cells, 2022 - mdpi.com
Adenosine 5′-triphosphate, or ATP, is the primary molecule for storing and transferring
energy in cells. ATP is mainly produced via oxidative phosphorylation in mitochondria, and …

Measurement of ATP concentrations in mitochondria of living cells using luminescence and fluorescence approaches

G Morciano, H Imamura, S Patergnani, G Pedriali… - Methods in Cell …, 2020 - Elsevier
Abstract Adenosine 5′-triphosphate (ATP) is the central metabolite in the energy
metabolism of cells and is hydrolyzed to ADP and inorganic phosphate to provide free …

Dissecting the regulation and function of ATP at the single-cell level

J Zhang, X Han, Y Lin - PLoS Biology, 2018 - journals.plos.org
Regulation of cellular ATP level is critical for diverse biological processes and may be
defective in diseases such as cancer and mitochondrial disorders. While mitochondria play …

Imaging changes in the cytosolic ATP-to-ADP ratio

M Tantama, G Yellen - Methods in enzymology, 2014 - Elsevier
Adenosine triphosphate (ATP) is a central metabolite that plays fundamental roles as an
energy transfer molecule, a phosphate donor, and a signaling molecule inside the cells. The …

Approaches to monitor ATP levels in living cells: where do we stand?

S Ley‐Ngardigal, G Bertolin - The FEBS Journal, 2022 - Wiley Online Library
ATP is the most universal and essential energy molecule in cells. This is due to its ability to
store cellular energy in form of high‐energy phosphate bonds, which are extremely stable …

Imaging extracellular ATP with a genetically-encoded, ratiometric fluorescent sensor

JM Conley, S Radhakrishnan, SA Valentino… - PLoS …, 2017 - journals.plos.org
Extracellular adenosine triphosphate (ATP) is a key purinergic signal that mediates cell-to-
cell communication both within and between organ systems. We address the need for a …

Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio

M Tantama, JR Martínez-François, R Mongeon… - Nature …, 2013 - nature.com
The ATP: ADP ratio is a critical parameter of cellular energy status that regulates many
metabolic activities. Here we report an optimized genetically encoded fluorescent biosensor …

Use of genetically encoded sensors to monitor cytosolic ATP/ADP ratio in living cells

AI Tarasov, GA Rutter - Methods in enzymology, 2014 - Elsevier
ATP is not only recognized as the universal energy “currency” in most cells but also plays a
less well-known role as an intracellular and extracellular messenger. Here, we review novel …

Cell-surface-anchored ratiometric DNA nanoswitch for extracellular ATP imaging

J Yuan, Z Deng, H Liu, X Li, J Li, Y He, Z Qing… - ACS …, 2019 - ACS Publications
The precise detection of extracellular ATP, although a challenging task, is of great
significance for understanding the related cell processes. Herein, we developed a …

Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor

N Kitajima, K Takikawa, H Sekiya, K Satoh, D Asanuma… - Elife, 2020 - elifesciences.org
Adenosine 5'triphosphate (ATP) is a ubiquitous extracellular signaling messenger. Here, we
describe a method for in-vivo imaging of extracellular ATP with high spatiotemporal …