There is growing interest in engineering Pseudomonas putida KT2440 as a microbial
chassis for the conversion of renewable and waste-based feedstocks, and metabolic …

Bacterial gene expression is orchestrated by numerous transcription factors (TFs).
Elucidating how gene expression is regulated is fundamental to understanding bacterial …

Pseudomonas putida KT2440 has long been studied for its diverse and robust metabolisms,
yet many genes and proteins imparting these growth capacities remain uncharacterized …

Randomly barcoded transposon insertion sequencing (RB-TnSeq) is an efficient,
multiplexed method to determine microbial gene function during growth under a selection …

Genomic integration is commonly used to engineer stable production hosts. However, so far,
for many microbial workhorses, only a few integration sites have been characterized …

Despite intensive study for 50years, the biochemical and genetic links between lysine
metabolism and central metabolism in Pseudomonas putida remain unresolved. To …

The environmental bacterium Pseudomonas putida is an organism endowed with a versatile
metabolism and stress tolerance traits that are desirable in an efficient production organism …

Thousands of Pseudomonas aeruginosa RNA sequencing (RNA-seq) gene expression
profiles are publicly available via the National Center for Biotechnology Information (NCBI) …

In silico models for Pseudomonas putida KT2440 metabolism predict 68 genes to be
essential for growth on minimal medium. In this study a genome‐wide collection of single …

A grand challenge in microbial ecology is disentangling the traits of individual populations
within complex communities. Various cultivation-independent approaches have been used …