We evaluated the usefulness and robustness of Agrobacterium tumefaciens-mediated
transformation (ATMT) as a high-throughput transformation tool for pathogenicity gene …

Leptosphaeria maculans, a fungal pathogen of Brassica spp., was successfully transformed
with the vector pAN8-1, encoding phleomycin resistance. Protoplasts of a vigorous Phleo r …

Background Many plant-pathogenic fungi have a tendency towards genome size expansion,
mostly driven by increasing content of transposable elements (TEs). Through comparative …

Agrobacterium tumefaciens-mediated random mutagenesis was used to generate
insertional mutants of the fungus Leptosphaeria maculans. Of 91 transformants screened …

Four filamentous ascomycetes, Leptosphaeria maculans, L. biglobosa, Oculimacula
yallundae and O. acuformis, were transformed via Agrobacterium tumefaciens-mediated …

Leptosphaeria maculans, a Dothideomycete causing stem canker on oilseed rape (Brassica
napus), develops gene‐for‐gene interactions with its host plants. To date, nine resistance …

Leptosphaeria maculans, the causal agent of stem canker of oilseed rape (Brassica napus),
develops gene-for-gene interactions with oilseed rape, and four L. maculans avirulence …

Recent comparisons of the increasing number of genome sequences have revealed that
variation in gene content is considerably more prevalent than previously thought. This …

Horizontal gene transfer (HGT) is a major driving force of evolution and is also likely to play
an important role in the threatening emergence of novel pathogens, especially if it involves …

Random insertional mutagenesis using a marker DNA fragment is an effective method for
identifying fungal genes relevant to morphogenesis, metabolism, and so on. Agrobacterium …