The proteome project, initiated in 1995, was made possible by 2‐DE combined with MS. The
project main objective was and remains the identification of all proteins expressed by a cell …

The proteome analysis by 2‐DE is one of the most potent methods of analyzing the complete
proteome of cells, cell lines, organs and tissues in proteomics studies. It allows a fast …

The need for methods to identify disease biomarkers is underscored by the survival-rate of
patients diagnosed at early stages of cancer progression. Surface enhanced laser …

Cancer can be defined as a deviated protein network system toward dysregulated cellular
proliferation. Alteration in the content and functional state of the proteins with many linkages …

Proteomics has its origins in two-dimensional gel electrophoresis (2-DE), a technique
developed more than twenty years ago. 2-DE has a high-resolution capacity, and was …

Significant technological advances in protein chemistry, physics and computer sciences in
the last two decades have greatly improved protein separation methodologies, such as …

Using more reliable and sophisticated protein biochemical techniques, it is possible to
perform large scale, partly high-throughput characterization of the human proteome. Two …

The main intricacy in the human proteome is that it is tremendously complex and composed
from diverse and heterogeneous gene products. These products are called protein species …

Proteomic research has proved valuable for understanding the molecular mechanisms of
biological processes, as well as in the search for biomarkers for a variety of diseases which …

Substantial advances have been made in the fundamental understanding of human biology,
ranging from DNA structure to identification of diseases associated with genetic …