[HTML][HTML] Effect of human testicular cells conditioned medium on in vitro maturation and morphology of mouse oocytes

M Adib, SM Seifati, MD Ashkezari… - … journal of fertility & …, 2020 - ncbi.nlm.nih.gov
Background Testicular cell conditioned medium (TCCM) has been shown to induce female
germ cell development in vitro from embryonic stem cells (ESCs). Testicular cells (TCs) …

Testicular cell–conditioned medium supports embryonic stem cell differentiation toward germ lineage and to spermatocyte-and oocyte-like cells

SM Shah, N Saini, MK Singh, R Manik, SK Singla… - Theriogenology, 2016 - Elsevier
Testicular cells are believed to secrete various growth factors that activate signaling
pathways finally leading to gametogenesis. In vitro gametogenesis is an obscure but …

[HTML][HTML] The effect of the human cumulus cells-conditioned medium on in vitro maturation of mouse oocyte: An experimental study

M Adib, SM Seifati, MD Ashkezari… - International Journal …, 2020 - ncbi.nlm.nih.gov
Background To increase the results of infertility treatment, many efforts have been made to
improve the treatment methods. As assisted reproductive technology is mainly using cell …

Testicular cell conditioned medium supports differentiation of embryonic stem cells into ovarian structures containing oocytes

O Lacham-Kaplan, H Chy, A Trounson - Stem cells, 2006 - academic.oup.com
Previous reports and the current study have found that germ cell precursor cells appear in
embryoid bodies (EBs) formed from mouse embryonic stem cells as identified by positive …

Coaxing HESC to form oocyte-like structures by co-culture with testicular extract and hormones

J Hua, BE Tuch, KS Sidhu - The Open Stem Cell Journal, 2011 - benthamopen.com
Reports have shown that murine embryonic stem cells (mESC) can differentiate into
primordial germ cells (PGC) and then to early gametes (oocytes and sperm) that after …

[PDF][PDF] The effect of conditioned media on human oocyte maturation and developmental competence

H Akbari, SHE Vaghefi, A Shahedi… - …, 2017 - researchgate.net
Aim: Oocyte cytoplasmic maturation is very important during in vitro maturation (IVM) and the
finding of an IVM medium that provides oocyte competence is very critical in assisted …

Comparison of different in vitro differentiation conditions for murine female germline stem cells

K Zou, J Wang, H Bi, Y Zhang, X Tian, N Tian… - Cell …, 2019 - Wiley Online Library
Objectives In vitro differentiation of oocytes from female germline stem cells (FGSCs) has
exciting potential applications for reproductive medicine. Some researchers have attempted …

[HTML][HTML] In vitro maturation (IVM) of murine and human germinal vesicle (GV)–stage oocytes by coculture with immortalized human fallopian tube epithelial cells

HJ Lee, AM Quaas, DL Wright, TL Toth, JM Teixeira - Fertility and sterility, 2011 - Elsevier
OBJECTIVE: To improve the maturation rate of murine and human germinal vesicle (GV)
oocytes using human tubal epithelial cells (hTECs). DESIGN: Murine and human GV …

Blastocyst development after assisted reproduction using spermatozoa obtained after testicular stem cell transplantation in mice

E Goossens, V Frederickx, G De Block… - Human …, 2006 - academic.oup.com
BACKGROUND: Since its introduction in 1994, testicular stem cell transplantation (TSCT)
has been widely used for research. This technique may also become important for …

[PDF][PDF] In Vitro Maturation of Human Oocytes using Conditioned Medium of Mesenchymal Stem Cells and Formation of Embryo by Use of ICSI

M Ghiasi, H Fazaely, E Asaii, M Sheykhhasan - Vitro, 2014 - academia.edu
Problems: In vitro maturation (IVM) of oocytes is a promising technique to reduce the costs
and avert the side-effects of gonadotrophin stimulation in in-vitro fertilization (IVF). On the …