Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing
approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre …

Embryogenic tissues of hybrid firs (Abies alba× A. cephalonica, Abies alba× A. numidica)
have been cryopreserved using a slow-freezing method. The cryotolerance of six cell lines …

Somatic embryogenesis of Abies alba (Mill.) has significant potential to become an effective
method for vegetative propagation of this species. To induce somatic embryogenesis in A …

Embryogenic tissues of hybrid firs were cryopreserved using a slow freezing protocol. The
procedure involved preculture of tissues for 24, 48 or 72 h in media with different sorbitol …

Abstract Cryopreservation (liquid nitrogen,− 196° C) is the only safe and cost-effective option
for long-term conservation of genetic resources of non-orthodox seed species …

The aim of the present study was to apply cryopreservation for the embryogenic cultures of
open-pollinated Abies cephalonica, as well as to test the genetic fidelity of the cryopreserved …

As a consequence of the difficulty in conventional coffee seed storage, biotechnological
alternatives such as cryopreservation have been investigated. The objective of this study …

Petiveria alliacea L. is a medicinal plant originating from the Amazon region. This study
describes an efficient cryopreservation protocol for somatic embryos (SEs) produced from …

This study was carried out to establish a suitable method for the cryopreservation of somatic
embryos of the herbaceous peony. The somatic embryos were obtained from cotyledon and …

Since its development in the 1960s, plant cryopreservation is considered an extraordinary
method of safe long-term conservation of biological material, as it does not induce genetic …