[HTML][HTML] Comparison of Nanopore and Synthesis-Based Next-Generation Sequencing Platforms for SARS-CoV-2 Variant Monitoring in Wastewater

D Garcia-Pedemonte, A Carcereny, J Gregori… - International Journal of …, 2023 - mdpi.com
Shortly after the beginning of the SARS-CoV-2 pandemic, many countries implemented
sewage sentinel systems to monitor the circulation of the virus in the population. A …

[HTML][HTML] SARS-CoV-2 whole-genome sequencing using Oxford nanopore technology for variant monitoring in wastewaters

L Barbé, J Schaeffer, A Besnard, S Jousse… - Frontiers in …, 2022 - frontiersin.org
Since the beginning of the Coronavirus Disease-19 (COVID-19) pandemic, multiple Severe
Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) mutations have been reported …

[HTML][HTML] Key SARS-CoV-2 mutations of alpha, gamma, and eta variants detected in urban wastewaters in Italy by long-read amplicon sequencing based on nanopore …

G La Rosa, D Brandtner, P Mancini, C Veneri… - Water, 2021 - mdpi.com
The emergence of SARS-CoV-2 variants of concern (VOCs) and variants of interest (VOIs)
poses an increased risk to global public health and underlines the need to prioritise …

High-resolution and real-time wastewater viral surveillance by Nanopore sequencing

X Xu, Y Deng, J Ding, Q Tang, Y Lin, X Zheng, T Zhang - Water Research, 2024 - Elsevier
Wastewater genomic sequencing stands as a pivotal complementary tool for viral
surveillance in populations. While long-read Nanopore sequencing is a promising platform …

[HTML][HTML] Long-term monitoring of SARS-CoV-2 variants in wastewater using a coordinated workflow of droplet digital PCR and nanopore sequencing

K Vigil, N D'Souza, J Bazner, FMA Cedraz, S Fisch… - Water Research, 2024 - Elsevier
Quantitative polymerase chain reaction (PCR) and genome sequencing are important
methods for wastewater surveillance of severe acute respiratory syndrome coronavirus 2 …

[HTML][HTML] Looking for a needle in a haystack. SARS-CoV-2 variant characterization in sewage

M Itarte, S Bofill-Mas, S Martínez-Puchol… - Current opinion in …, 2021 - Elsevier
SARS-CoV-2 variants are emerging worldwide and monitoring them is key in providing early
warnings. Here, we summarize the different analytical approaches currently used to study …

Direct comparison of RT-ddPCR and targeted amplicon sequencing for SARS-CoV-2 mutation monitoring in wastewater

EG Lou, N Sapoval, C McCall, L Bauhs… - Science of The Total …, 2022 - Elsevier
Over the course of the COVID-19 pandemic, variants of SARS-CoV-2 have emerged that are
more contagious and more likely to cause breakthrough infections. Targeted amplicon …

[HTML][HTML] VirPool: model-based estimation of SARS-CoV-2 variant proportions in wastewater samples

A Gafurov, A Baláž, F Amman, K Boršová… - BMC …, 2022 - Springer
Background The genomes of SARS-CoV-2 are classified into variants, some of which are
monitored as variants of concern (eg the Delta variant B. 1.617. 2 or Omicron variant B. 1.1 …

[HTML][HTML] Interlaboratory comparison using inactivated SARS-CoV-2 variants as a feasible tool for quality control in COVID-19 wastewater monitoring

A Wilhelm, J Schoth, C Meinert-Berning… - Science of the Total …, 2023 - Elsevier
Abstract Wastewater-based SARS-CoV-2 epidemiology (WBE) has proven as an excellent
tool to monitor pandemic dynamics supporting individual testing strategies. WBE can also be …

[HTML][HTML] Detecting SARS-CoV-2 lineages and mutational load in municipal wastewater and a use-case in the metropolitan area of Thessaloniki, Greece

N Pechlivanis, M Tsagiopoulou, MC Maniou… - Scientific reports, 2022 - nature.com
The COVID-19 pandemic represents an unprecedented global crisis necessitating novel
approaches for, amongst others, early detection of emerging variants relating to the …