The strategy of rational design to engineer enzymes is to predict the potential mutants based
on the understanding of the relationships between protein structure and function, and …

Abstract Enzymes offering chemo-, regio-, and stereoselectivity enable the asymmetric
synthesis of high-value chiral molecules. Unfortunately, the drawback that naturally …

This mini review gives an overview over different design approaches and methodologies
applied in rational and semirational enzyme engineering. The underlying principles for …

With the development of enzyme engineering technology, green enzyme catalysis is widely
poised to replace traditional chemical catalysis enabling high enantioselectivity and yielding …

This review outlines recent developments in protein engineering of stereo‐and
regioselective enzymes, which are of prime interest in organic and pharmaceutical chemistry …

Site-directed mutagenesis is still a very efficient strategy to elaborate improved enzymes.
Recently, advances have been made in developing rational strategies aimed at reshaping …

Enzymes are an attractive alternative in the asymmetric syntheses of chiral building blocks.
To meet the requirements of industrial biotechnology and to introduce new functionalities …

Computational protein design relies on several approximations, including the use of fixed
backbones and rotamers, to reduce protein design to a computationally tractable problem …

With advances in protein structure predictions, the number of available high-quality
structures has increased dramatically. In light of these advances, structure-based enzyme …

The challenging field of de novo enzyme design is beginning to produce exciting results.
The application of powerful computational methods to functional protein design has recently …