Type IV secretion system (T4SS) substrates are recruited through a translocation signal that
is poorly defined for conjugative relaxases. The relaxase TrwC of plasmid R388 is …

Bacterial conjugation, a mechanism of horizontal gene transfer, is the major means by which
antibiotic resistance spreads among bacteria (1, 2). Conjugative plasmids are transferred …

Bacterial type IV secretion systems (T4SSs) are involved in processes such as bacterial
conjugation and protein translocation to animal cells. In this work, we have switched the …

In preparation for transfer conjugative type IV secretion systems (T4SS) produce a
nucleoprotein adduct containing a relaxase enzyme covalently linked to the 5′ end of …

Relaxases are proteins responsible for the transfer of plasmid and chromosomal DNA from
one bacterium to another during conjugation. They covalently react with a specific …

Conjugative coupling proteins (CPs) are proposed to play a role in connecting the
relaxosome to a type IV secretion system (T4SS) during bacterial conjugation. Here we …

Bacterial type IV secretion (T4S) systems mediate the transfer of macromolecular substrates
into various target cells, eg, the conjugative transfer of DNA into bacteria or the transfer of …

A large subfamily of the type IV secretion systems (T4SSs), termed the conjugation systems,
transmit mobile genetic elements (MGEs) among many bacterial species. In the initiating …

The conjugative coupling protein TrwB is responsible for connecting the relaxosome to the
type IV secretion system during conjugative DNA transfer of plasmid R388. It is directly …

Bacterial conjugation is a mechanism of horizontal DNA transfer. The relaxase TrwC of the
conjugative plasmid R388 cleaves one strand of the transferred DNA at the oriT gene …