Development and applications of a CRISPR activation system for facile genetic overexpression in Candida albicans
For the fungal pathogen Candida albicans, genetic overexpression readily occurs via a
diversity of genomic alterations, such as aneuploidy and gain-of-function mutations, with …
diversity of genomic alterations, such as aneuploidy and gain-of-function mutations, with …
[PDF][PDF] Implementation of a CRISPR-based system for gene regulation in Candida albicans. mSphere 4: e00001-19
E Roman, I Coman, D Prieto, R Alonso-Monge, J Pla… - 2019 - docta.ucm.es
Clustered regularly interspaced short palindromic repeat (CRISPR) methodology is not only
an efficient tool in gene editing but also an attractive platform to facilitate DNA, RNA, and …
an efficient tool in gene editing but also an attractive platform to facilitate DNA, RNA, and …
Implementation of a CRISPR-Based System for Gene Regulation in Candida albicans
Clustered regularly interspaced short palindromic repeat (CRISPR) methodology is not only
an efficient tool in gene editing but also an attractive platform to facilitate DNA, RNA, and …
an efficient tool in gene editing but also an attractive platform to facilitate DNA, RNA, and …
[PDF][PDF] A CRISPR interference platform for efficient genetic repression in Candida albicans. mSphere 4: e00002-19
L Wensing, J Sharma, D Uthayakumar, Y Proteau… - 2019 - academia.edu
Fungal pathogens are emerging as an important cause of human disease, and Candida
albicans is among the most common causative agents of fungal infections. Studying this …
albicans is among the most common causative agents of fungal infections. Studying this …
A CRISPR Interference Platform for Efficient Genetic Repression in Candida albicans
L Wensing, J Sharma, D Uthayakumar, Y Proteau… - Msphere, 2019 - Am Soc Microbiol
Fungal pathogens are emerging as an important cause of human disease, and Candida
albicans is among the most common causative agents of fungal infections. Studying this …
albicans is among the most common causative agents of fungal infections. Studying this …
A Candida albicans CRISPR system permits genetic engineering of essential genes and gene families
VK Vyas, MI Barrasa, GR Fink - Science advances, 2015 - science.org
Candida albicans is a pathogenic yeast that causes mucosal and systematic infections with
high mortality. The absence of facile molecular genetics has been a major impediment to …
high mortality. The absence of facile molecular genetics has been a major impediment to …
Targeted Genetic Changes in Candida albicans Using Transient CRISPR‐Cas9 Expression
MY Huang, MV Cravener, AP Mitchell - Current protocols, 2021 - Wiley Online Library
Candida albicans is an opportunistic fungal pathogen responsible for significant disease
and mortality. Absent complete mating and other convenient methods, dissection of its …
and mortality. Absent complete mating and other convenient methods, dissection of its …
Candida albicans gene deletion with a transient CRISPR-Cas9 system
K Min, Y Ichikawa, CA Woolford, AP Mitchell - MSphere, 2016 - Am Soc Microbiol
ABSTRACT C lustered r egularly i nterspaced s hort p alindromic r epeat (CRISPR) and
CRISPR-associated gene 9 (CRISPR-Cas9) systems are used for a wide array of genome …
CRISPR-associated gene 9 (CRISPR-Cas9) systems are used for a wide array of genome …
Design, execution, and analysis of CRISPR–Cas9-based deletions and genetic interaction networks in the fungal pathogen Candida albicans
The study of fungal pathogens is of immediate importance, yet progress is hindered by the
technical challenges of genetic manipulation. For Candida species, their inability to maintain …
technical challenges of genetic manipulation. For Candida species, their inability to maintain …
A Modular Cloning Toolkit Including CRISPRi for the Engineering of the Human Fungal Pathogen and Biotechnology Host Candida glabrata
S Billerbeck, RC Prins, M Marquardt - ACS Synthetic Biology, 2023 - ACS Publications
The yeast Candida glabrata is an emerging, often drug-resistant opportunistic human
pathogen that can cause severe systemic infections in immunocompromised individuals. At …
pathogen that can cause severe systemic infections in immunocompromised individuals. At …