[HTML][HTML] Evaluation of Sorghum [Sorghum bicolor (L.)] Reference Genes in Various Tissues and under Abiotic Stress Conditions for Quantitative Real-Time PCR Data …

P Sudhakar Reddy, D Srinivas Reddy… - Frontiers in plant …, 2016 - frontiersin.org
Accurate and reliable gene expression data from qPCR depends on stable reference gene
expression for potential gene functional analyses. In this study, 15 reference genes were …

[HTML][HTML] Selection of reliable reference genes for quantitative real-time PCR gene expression analysis in Jute (Corchorus capsularis) under stress treatments

X Niu, J Qi, G Zhang, J Xu, A Tao, P Fang… - Frontiers in plant …, 2015 - frontiersin.org
To accurately measure gene expression using quantitative reverse transcription PCR (qRT-
PCR), reliable reference gene (s) are required for data normalization. Corchorus capsularis …

[HTML][HTML] Identification and Validation of Reference Genes and Their Impact on Normalized Gene Expression Studies across Cultivated and Wild Cicer Species

DS Reddy, P Bhatnagar-Mathur, PS Reddy… - PloS one, 2016 - journals.plos.org
Quantitative Real-Time PCR (qPCR) is a preferred and reliable method for accurate
quantification of gene expression to understand precise gene functions. A total of 25 …

[HTML][HTML] Selection and Validation of Reference Genes for Quantitative Real-Time PCR in Buckwheat (Fagopyrum esculentum) Based on Transcriptome Sequence …

NV Demidenko, MD Logacheva, AA Penin - PloS one, 2011 - journals.plos.org
Quantitative reverse transcription PCR (qRT-PCR) is one of the most precise and widely
used methods of gene expression analysis. A necessary prerequisite of exact and reliable …

[HTML][HTML] Selection of reference genes for qPCR analyses of gene expression in ramie leaves and roots across eleven abiotic/biotic treatments

Y Yu, G Zhang, Y Chen, Q Bai, C Gao, L Zeng, Z Li… - Scientific reports, 2019 - nature.com
Quantitative real-time PCR (qPCR) is commonly used for deciphering gene functions. For
effective qPCR analyses, suitable reference genes are needed for normalization. The …

Validation of reference genes for RT-qPCR normalization in common bean during biotic and abiotic stresses

A Borges, SM Tsai, DGG Caldas - Plant cell reports, 2012 - Springer
Selection of reference genes is an essential consideration to increase the precision and
quality of relative expression analysis by the quantitative RT-PCR method. The stability of …

[HTML][HTML] Recommended reference genes for quantitative PCR analysis in soybean have variable stabilities during diverse biotic stresses

R Bansal, P Mittapelly, BJ Cassone, P Mamidala… - PloS one, 2015 - journals.plos.org
For real-time reverse transcription-PCR (qRT-PCR) in soybean, reference genes in different
tissues, developmental stages, various cultivars, and under stress conditions have been …

[HTML][HTML] Stability evaluation of reference genes for gene expression analysis by RT-qPCR in soybean under different conditions

Q Wan, S Chen, Z Shan, Z Yang, L Chen, C Zhang… - PloS one, 2017 - journals.plos.org
Real-time quantitative reverse transcription PCR is a sensitive and widely used technique to
quantify gene expression. To achieve a reliable result, appropriate reference genes are …

[HTML][HTML] Evaluation of appropriate reference genes for reverse transcription-quantitative PCR studies in different tissues of a desert poplar via comparision of different …

HL Wang, L Li, S Tang, C Yuan, Q Tian, Y Su… - International journal of …, 2015 - mdpi.com
Despite the unshakable status of reverse transcription-quantitative PCR in gene expression
analysis, it has certain disadvantages, including that the results are highly dependent on the …

[HTML][HTML] Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative RT-PCR

H Ling, Q Wu, J Guo, L Xu, Y Que - PloS one, 2014 - journals.plos.org
The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method
for gene expression analysis requires one or several reference gene (s) acting as …