Improved protocol for Bst polymerase and reverse transcriptase production and application to a point-of-care diagnostics system

LR de Souza, IEP Silva, G Celis-Silva… - Experimental …, 2023 - journals.sagepub.com
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
has raised awareness in the scientific community about the importance of being prepared for …

[HTML][HTML] Homebrew reagents for low-cost RT-LAMP

T Matute, I Nuñez, M Rivera, J Reyes… - Journal of …, 2021 - ncbi.nlm.nih.gov
Reverse transcription–loop-mediated isothermal amplification (RT-LAMP) has gained
popularity for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV …

Starting from scratch: Step-by-step development of diagnostic tests for SARS-CoV-2 detection by RT-LAMP

DA Tapia-Sidas, BY Vargas-Hernández… - Plos one, 2023 - journals.plos.org
The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-
2) has affected millions of people worldwide. Public health strategies to reduce viral …

Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples

A Alekseenko, D Barrett, Y Pareja-Sanchez… - Scientific reports, 2021 - nature.com
Abstract RT-LAMP detection of SARS-CoV-2 has been shown to be a valuable approach to
scale up COVID-19 diagnostics and thus contribute to limiting the spread of the disease …

Sample Treatment with Trypsin for RT-LAMP COVID-19 Diagnosis

S García-Sorribes, F Lara-Hernández… - Biology, 2023 - mdpi.com
Simple Summary At the end of 2019, a new virus named SARS-CoV-2, which causes COVID-
19 disease, emerged. It was recognized as a pandemic on 11 March 2020 and caused the …

Development of an optimized colorimetric RT-LAMP for SARS-CoV-2 assay with enhanced procedure controls for remote diagnostics

BW Raddatz, EYS Kim, LM Imamura, GJ Steil… - Scientific Reports, 2022 - nature.com
The coronavirus pandemic accentuated the need for molecular diagnostic tests. A technique
highly used to this end is the Polymerase Chain Reaction (PCR)—a sensitive and specific …

Sequence-specific and multiplex detection of COVID-19 virus (SARS-CoV-2) using proofreading enzyme-mediated probe cleavage coupled with isothermal …

S Ding, G Chen, Y Wei, J Dong, F Du, X Cui… - Biosensors and …, 2021 - Elsevier
The outbreak of COVID-19 caused by severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) has been challenging human health worldwide. Loop-mediated isothermal …

Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection

Y Nakura, HN Wu, Y Okamoto, M Takeuchi, K Suzuki… - PLoS …, 2021 - journals.plos.org
The general methods to detect the RNA of severe acute respiratory syndrome-coronavirus 2
(SARS-CoV-2) in clinical diagnostic testing involve reverse transcriptases and thermostable …

Quick and easy assembly of a one-step qRT-PCR Kit for COVID-19 diagnostics using in-house enzymes

M Takahashi, M Tehseen, R Salunke, E Takahashi… - ACS …, 2021 - ACS Publications
One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is the
most widely applied method for COVID-19 diagnostics. Notwithstanding the facts that one …

[HTML][HTML] Corona Detective: a simple, scalable, and robust SARS-CoV-2 detection method based on reverse transcription loop-mediated isothermal amplification

G Aidelberg, R Aronoff, T Eliseeva… - Journal of …, 2021 - ncbi.nlm.nih.gov
Surveillance screening at scale to identify people infected by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) prior to extensive transmission is key to bringing an …