High-stearic and high-oleic cottonseed oils produced by hairpin RNA-mediated post-transcriptional gene silencing

Q Liu, SP Singh, AG Green - Plant physiology, 2002 - academic.oup.com
We have genetically modified the fatty acid composition of cottonseed oil using the recently
developed technique of hairpin RNA-mediated gene silencing to down-regulate the seed …

High-oleic and high-stearic cottonseed oils: nutritionally improved cooking oils developed using gene silencing

Q Liu, S Singh, A Green - Journal of the american College of …, 2002 - Taylor & Francis
Gene technology and plant breeding are combining to provide powerful means for modifying
the composition of oilseeds to improve their nutritional value and provide the functional …

Transgenic cotton plants with increased seed oleic acid content

KD Chapman, S Austin‐Brown… - Journal of the …, 2001 - Wiley Online Library
Cottonseed typically contains about 15% oleic acid. Here we report the development of
transgenic cotton plants with higher seed oleic acid levels. Plants were generated by …

Simultaneous silencing of FAD2 and FAE1 genes affects both oleic acid and erucic acid contents in Brassica napus seeds

Q Peng, Y Hu, R Wei, Y Zhang, C Guan, Y Ruan, C Liu - Plant cell reports, 2010 - Springer
The fatty acid composition in the seed oil was significantly modified following the
introduction of transgenes. To further enhance the desirable characteristics of rapeseed oil …

Seed-specific expression of sesame microsomal oleic acid desaturase is controlled by combinatorial properties between negative cis-regulatory elements in the …

MJ Kim, H Kim, JS Shin, CH Chung… - Molecular Genetics and …, 2006 - Springer
The regulation of genes involved in primary lipid metabolism in plants is much less well
understood than that in many other pathways in plant biology. In the investigation reported …

Gene silencing of Sugar-dependent 1 (JcSDP1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in Jatropha curcas

MJ Kim, SW Yang, HZ Mao, SP Veena, JL Yin… - Biotechnology for …, 2014 - Springer
Abstract Background Triacylglycerols (TAGs) are the most abundant form of storage oil in
plants. They consist of three fatty acid chains (usually C16 or C18) covalently linked to …

Silencing of GmFAD3 gene by siRNA leads to low α-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)]

T Flores, O Karpova, X Su, P Zeng, K Bilyeu… - Transgenic …, 2008 - Springer
RNA interference (RNAi) has been recently employed as an effective experimental tool for
both basic and applied biological studies in various organisms including plants. RNAi …

A high‐throughput screen for genes from castor that boost hydroxy fatty acid accumulation in seed oils of transgenic Arabidopsis

C Lu, M Fulda, JG Wallis, J Browse - The Plant Journal, 2006 - Wiley Online Library
It is desirable to produce high homogeneity of novel fatty acids in oilseeds through genetic
engineering to meet the increasing demands of the oleo‐chemical industry. However …

Ribozyme termination of RNA transcripts down‐regulate seed fatty acid genes in transgenic soybean

T Buhr, S Sato, F Ebrahim, A Xing, Y Zhou… - The Plant …, 2002 - Wiley Online Library
We investigated whether termination of transcripts with a self‐cleaving ribozyme can
enhance nuclear retention and serve as a tool to decrease specific plant gene expression …

Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene.

DS Knutzon, GA Thompson… - Proceedings of the …, 1992 - National Acad Sciences
Molecular gene transfer techniques have been used to engineer the fatty acid composition
of Brassica rapa and Brassica napus (canola) oil. Stearoyl-acyl carrier protein (stearoyl …