The metabolic redox regime of Pseudomonas putida tunes its evolvability toward novel xenobiotic substrates

Ö Akkaya, DR Pérez-Pantoja, B Calles, PI Nikel… - MBio, 2018 - Am Soc Microbiol
During evolution of biodegradation pathways for xenobiotic compounds involving Rieske
nonheme iron oxygenases, the transition toward novel substrates is frequently associated …

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

PI Nikel, T Fuhrer, M Chavarría… - The ISME …, 2021 - academic.oup.com
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …

Metabolic networks to combat oxidative stress in Pseudomonas fluorescens

RJ Mailloux, J Lemire, VD Appanna - Antonie Van Leeuwenhoek, 2011 - Springer
Oxidative stress is an unavoidable peril that aerobic organisms have to confront. Thus, it is
not surprising that intricate strategies are deployed in an effort to fend the dangers …

Elucidating the Role of O2 Uncoupling for the Adaptation of Bacterial Biodegradation Reactions Catalyzed by Rieske Oxygenases

CE Bopp, NM Bernet, F Meyer, R Khan… - ACS …, 2023 - ACS Publications
Oxygenation of aromatic and aliphatic hydrocarbons by Rieske oxygenases is the initial step
of various biodegradation pathways for environmental organic contaminants …

Response of Pseudomonas putida KT2440 to increased NADH and ATP demand

BE Ebert, F Kurth, M Grund, LM Blank… - Applied and …, 2011 - Am Soc Microbiol
Adenosine phosphate and NAD cofactors play a vital role in the operation of cell
metabolism, and their levels and ratios are carefully regulated in tight ranges. Perturbations …

Pyridine nucleotide transhydrogenases enable redox balance of Pseudomonas putida during biodegradation of aromatic compounds

PI Nikel, D Pérez‐Pantoja… - Environmental …, 2016 - Wiley Online Library
The metabolic versatility of the soil bacterium Pseudomonas putida is reflected by its ability
to execute strong redox reactions (eg, mono‐and di‐oxygenations) on aromatic substrates …

Elucidating the Role of O2 Uncoupling in the Oxidative Biodegradation of Organic Contaminants by Rieske Non-heme Iron Dioxygenases

CE Bopp, NM Bernet, HPE Kohler… - ACS Environmental …, 2022 - ACS Publications
Oxygenations of aromatic soil and water contaminants with molecular O2 catalyzed by
Rieske dioxygenases are frequent initial steps of biodegradation in natural and engineered …

The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress

M Chavarría, PI Nikel, D Pérez‐Pantoja… - Environmental …, 2013 - Wiley Online Library
Glucose catabolism of P seudomonas putida is carried out exclusively through the E ntner–
D oudoroff (ED) pathway due to the absence of 6‐phosphofructokinase. In order to activate …

Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase

LM Blank, G Ionidis, BE Ebert, B Bühler… - The FEBS …, 2008 - Wiley Online Library
A key limitation of whole‐cell redox biocatalysis for the production of valuable, specifically
functionalized products is substrate/product toxicity, which can potentially be overcome by …

Coupling microbial catabolic actions with abiotic redox processes: A new recipe for persistent organic pollutant (POP) removal

JR Jeon, K Murugesan, IH Nam, YS Chang - Biotechnology Advances, 2013 - Elsevier
The continuous release of toxic persistent organic pollutants (POPs) into the environment
has raised a need for effective cleanup methods. The tremendous natural diversity of …