Conjugative DNA metabolism in Gram-negative bacteria

F De La Cruz, LS Frost, RJ Meyer… - FEMS microbiology …, 2010 - academic.oup.com
Bacterial conjugation in Gram-negative bacteria is triggered by a signal that connects the
relaxosome to the coupling protein (T4CP) and transferosome, a type IV secretion system …

DNA processing reactions in bacterial conjugation

E Lanka, BM Wilkins - Annual review of biochemistry, 1995 - annualreviews.org
Bacterial conjugation is an important source of genetic plasticity. The initiation complex for
conjugative transfer of transmissible plasmids—the relaxosome—is a specific DNA-protein …

Bacterial conjugation: a two‐step mechanism for DNA transport

M Llosa, FX Gomis‐Rüth, M Coll… - Molecular …, 2002 - Wiley Online Library
Bacterial conjugation is a promiscuous DNA transport mechanism. Conjugative plasmids
transfer themselves between most bacteria, thus being one of the main causal agents of the …

Conjugative transfer can be inhibited by blocking relaxase activity within recipient cells with intrabodies

MP Garcillán‐Barcia, P Jurado… - Molecular …, 2007 - Wiley Online Library
Horizontal transfer of antibiotic resistance genes carried by conjugative plasmids poses a
serious health problem. As conjugative relaxases are transported to recipient cells during …

Bacterial conjugation: a potential tool for genomic engineering

M Llosa, F de la Cruz - Research in Microbiology, 2005 - Elsevier
Bacterial conjugation is a mechanism for horizontal DNA transfer with potential for universal
DNA delivery. The conjugal machinery can be separated into three functional modules: the …

From conjugation to T4S systems in Gram‐negative bacteria: a mechanistic biology perspective

G Waksman - EMBO reports, 2019 - embopress.org
Conjugation is the process by which bacteria exchange genetic materials in a unidirectional
manner from a donor cell to a recipient cell. The discovery of conjugation signalled the dawn …

Structural biology of the Gram-negative bacterial conjugation systems

A Ilangovan, S Connery, G Waksman - Trends in microbiology, 2015 - cell.com
Conjugation, the process by which plasmid DNA is transferred from one bacterium to
another, is mediated by type IV secretion systems (T4SSs). T4SSs are versatile systems that …

Nicking by transesterification: the reaction catalysed by a relaxase

DR Byrd, SW Matson - Molecular microbiology, 1997 - Wiley Online Library
DNA relaxases play an essential role in the initiation and termination of conjugative DNA
transfer. Purification and characterization of relaxases from several plasmids has revealed …

Stepwise assembly of a relaxosome at the F plasmid origin of transfer

MT Howard, WC Nelson, SW Matson - Journal of Biological Chemistry, 1995 - ASBMB
A central step in the transfer of genetic information during bacterial conjugation of the
Escherichia coli F plasmid involves the formation of a protein-DNA complex, called the …

Conjugation in gram‐positive bacteria

N Goessweiner-Mohr, K Arends… - … : Biology and Impact …, 2015 - Wiley Online Library
Conjugative transfer is an important driver in evolution, enabling bacteria to acquire new
traits (1, 2, 3, 4, 5). During conjugative transfer, DNA translocation across the cell envelopes …