Microwave-assisted dehydration, long-term storage at non-freezing temperatures, and rehydration of cat germinal vesicles
PC Lee, P Comizzoli - Biology of Reproduction, 2024 - academic.oup.com
Germinal vesicles are alternative targets for female fertility preservation due to their
availability and high resilience against non-physiological conditions. Preserved germinal …
availability and high resilience against non-physiological conditions. Preserved germinal …
Resilience of oocyte germinal vesicles to microwave-assisted drying in the domestic cat model
GD Elliott, PC Lee, E Paramore… - Biopreservation and …, 2015 - liebertpub.com
The ability to compact and inject the cat germinal vesicle (GV) into a recipient cytoplast
allows exploration of a new fertility preservation strategy that avoids whole oocyte freezing …
allows exploration of a new fertility preservation strategy that avoids whole oocyte freezing …
Influence of microwave-assisted dehydration on morphological integrity and viability of cat ovarian tissues: First steps toward long-term preservation of complex …
PC Lee, DM Adams, O Amelkina, KK White… - PLoS …, 2019 - journals.plos.org
Ovarian tissue contains large pools of immature oocytes enclosed in primordial follicles,
making it an attractive target for fertility preservation in female cancer patients, livestock and …
making it an attractive target for fertility preservation in female cancer patients, livestock and …
Retention of structure and function of the cat germinal vesicle after air-drying and storage at suprazero temperature
JE Graves-Herring, DE Wildt… - Biology of …, 2013 - academic.oup.com
The study explored a novel approach for preserving the maternal genome without the entire
oocyte by air-drying the cat germinal vesicle (GV) in the presence of the disaccharide …
oocyte by air-drying the cat germinal vesicle (GV) in the presence of the disaccharide …
Desiccation and supra‐zero temperature storage of cat germinal vesicles lead to less structural damage and similar epigenetic alterations compared to …
PC Lee, P Comizzoli - Molecular reproduction and …, 2019 - Wiley Online Library
Understanding cellular and molecular damages in oocytes during exposure to extreme
conditions is essential to optimize long‐term fertility preservation approaches. Using the …
conditions is essential to optimize long‐term fertility preservation approaches. Using the …
[HTML][HTML] The Effect of Choline Salt Addition to Trehalose Solution for Long-Term Storage of Dried and Viable Nuclei from Fully Grown Oocytes
JAO Cabral, PC Lee, S Wang, Y Wang, Y Zhang… - …, 2023 - ncbi.nlm.nih.gov
Although drying techniques are exciting alternatives to cryopreservation, it remains
challenging to maintain tightly controlled temperatures and humidity levels during storage of …
challenging to maintain tightly controlled temperatures and humidity levels during storage of …
The Effect of Choline Salt Addition to Trehalose Solution for Long-Term Storage of Dried and Viable Nuclei from Fully Grown Oocytes
JA Orozco Cabral, PC Lee, S Wang, Y Wang, Y Zhang… - Bioengineering, 2023 - mdpi.com
Although drying techniques are exciting alternatives to cryopreservation, it remains
challenging to maintain tightly controlled temperatures and humidity levels during storage of …
challenging to maintain tightly controlled temperatures and humidity levels during storage of …
Preserving the female genome in trehalose glass at supra-zero temperatures: The relationship between moisture content and DNA damage in feline germinal vesicles
Introduction Maintaining a stable dry state is critical for long-term preservation of live
biomaterials at suprazero temperatures. The objective of the study was to characterize the …
biomaterials at suprazero temperatures. The objective of the study was to characterize the …
Long-term preservation of germ cells and gonadal tissues at ambient temperatures
Objective To present an overview of different approaches and recent advances for long-term
preservation of germ cells and gonadal tissues at ambient temperatures. Methods Review of …
preservation of germ cells and gonadal tissues at ambient temperatures. Methods Review of …
Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation
P Comizzoli, O Amelkina, PC Lee - Molecular Reproduction and …, 2022 - Wiley Online Library
Long‐term preservation of sperm, oocytes, and gonadal tissues at ambient temperatures
has the potential to lower the costs and simplify biobanking in human reproductive medicine …
has the potential to lower the costs and simplify biobanking in human reproductive medicine …
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