N-phosphorylation modifications are crucial for prokaryotic signal transduction and verified
as intermediate for several metabolic enzymes, yet the landscape of N-phosphorylation …

Protein phosphorylation is one of the most commonly studied and ubiquitous post-
translational modifications (PTMs), and defining site-specific phosphorylation is essential to …

Protein N-phosphorylation plays a critical role in central metabolism and
two/multicomponent signaling of prokaryotes. However, the current enrichment methods for …

Protein phosphorylation plays essential roles in various biological procedures. Despite the
well-established enrichment strategies for O-phosphoproteomics, the intrinsic acid lability of …

Many cellular processes are regulated by the reversible phosphorylation of proteins. Despite
the importance of monitoring protein phosphorylation, available methods to modify and …

蛋白质磷酸化作为一种最普遍和最重要的翻译后修饰调控着几乎所有的生命过程.
随着高效富集方法和生物质谱技术的快速发展, 低丰度的蛋白质 O-磷酸化修饰获得了规模化 …

Phosphorylation is a post-translational modification (PTM) fundamental for processes such
as signal transduction and enzyme activity. We propose to apply data-independent …

Phosphorylation is a reversible posttranslational protein modification which plays a pivotal
role in intracellular signaling. Despite extensive efforts, phosphorylation site mapping of …

Protein phosphorylation is a ubiquitous post-translational modification (PTM) that regulates
all aspects of life. To date, investigation of human cell signalling has focussed on canonical …

Investigating site-specific protein phosphorylation remains a challenging task. The present
study introduces a two-step chemical derivatization method for accurate identification of …