A modified procedure is reported that enables microtubule assembly to occur in extracts of
differentiated neuroblastoma cells. Of the proteins that co-assemble through five successive …

We have attempted a biochemical characterization of the microtubule-associated proteins
(MAPs) of cultured HeLa cells. The HeLa MAPs consist of a group of three polypeptides of …

Microtubule protein from HeLa cell extracts was purified by multiple cycles of polymerization
and depolymerization in the absence of glycerol or other exogenous polymerization …

Microtubules prepared from brain tissue by successive cycles of assembly and disassembly
contain, in addition to tubulin, several nontubulin proteins which will promote in vitro …

In this paper we describe a procedure for detecting proteins associated with cytoplasmic
microtubules in vivo. Detergent-extracted cytoskeletons of NIL8 hamster cells are prepared …

Eric J. Aamodt and Robley C. Williams, Jr.* abstract: Neuronal intermediate filaments
(neurofilaments) prepared from brain form a viscous, sedimentable complex with …

One of the major groups of microtubule-associated proteins (MAPs) found associated with
the microtubules isolated from HeLa cells has a molecular weight of just over 200,000 …

Roger D. Sloboda,*** § William L. Dentler, 11 and Joel L. Rosenbaum abstract:
Microtubules, purified bycycles of assembly and disassembly in vitro, are composed of …

We have analyzed the detailed structure and cytoplasmic distribution of cytoplasmic
microtubule-associated proteins. The procedure used to identify these proteins, based on …

Douglas B. Murphy, Richard B. Vallee,* and Gary G. Borisy* abstract: Microtubule protein
purified by an in vitro as-sembly procedure contains several accessory proteins which …