Mounting evidence suggests that transcription and RNA processing are intimately coupled
in vivo, although each process can occur independently in vitro. It is generally thought that …

Mammalian splicing factor SF1 consists of a single polypeptide of 75 kDa and is required for
the formation of the first ATP-dependent spliceosomal complex. Three cDNAs encoding …

SC35 belongs to the family of SR proteins that regulate alternative splicing in a
concentration‐dependent manner in vitro and in vivo. We previously reported that SC35 is …

Transcription controls splicing and other gene regulatory processes, yet mechanisms remain
obscure due to our fragmented knowledge of the molecular connections between the …

Abstract Splicing factor 1 (SF1) functions at early stages of pre‐mRNA splicing and
contributes to splice site recognition by interacting with the essential splicing factor U2AF65 …

Previous studies have linked the C-terminal domain (CTD) of RNA polymerase II (pol II) with
cotranscriptional precursor messenger RNA processing, but little is known about the CTD's …

Coupling between transcription and RNA processing is a key gene regulatory mechanism.
Here we use chromatin immunoprecipitation to detect transcription-dependent accumulation …

The cellular protein p32 was isolated originally as a protein tightly associated with the
essential splicing factor ASF/SF2 during its purification from HeLa cells. ASF/SF2 is a …

The mammalian splicing factor SC35 is required for the first step in the splicing reaction and
for spliceosome assembly. The cloning and characterization of a complementary DNA …

Promoters and enhancers are cis-acting elements that control gene transcription via
complex networks of protein–DNA and protein–protein interactions. Whereas promoters …