The aim of the study was to develop an effective cryopreservation method for Scots pine
(Pinus sylvestris L.) embryogenic cultures. Altogether nine cell lines derived from three …

Embryogenic cultures of chir pine (Pinus roxburghii Sarg.) were cryopreserved successfully
in liquid nitrogen. It was found that using sorbitol and dimethyl sulfoxide (DMSO) as …

Embryogenic cultures were obtained from seeds of open-pollinated maritime pine trees
representing part of a breeding population. The aim of the present study was to develop and …

Embryogenic tissues of a conifer species Pinus nigra Arn. have been cryopreserved by a
slow-freezing method. The effect of cryoprotective compounds such as maltose, sucrose or …

Six different embryogenic cell lines of Pinus nigra Arn. have been cryopreserved in liquid
nitrogen using cryoprotection with sucrose (18%) and DMSO (7.5%). Post-thaw growth and …

Embryogenic cultures of interior spruce derived from 12 full-sib families were subjected to
cryopreservation, with a 97% success rate for 357 genotypes. Analyses suggested that …

This study reports on the first use of a vitrification procedure for the successful
cryopreservation of embryogenic cultures of a coniferous species. Using Picea mariana …

Embryogenic tissue of Pinus patula Scheide et Deppe was cryopreserved for 8 weeks using
sorbitol and dimethylsulfoxide (DMSO) as cryoprotectants. Results indicate that 0.3 M …

A vitrification method enabled efficient cryopreservation of embryogenic tissue (ETs) of
Norway spruce (Picea abies L.) at− 196° C in liquid nitrogen (LN). Correctly formed, normal …

Cryopreservation of embryogenic tissue is an essential storage step in genotype selection
and seedling production through somatic embryogenesis. To date, immature conifer somatic …