Toward quantitative metabarcoding
AO Shelton, ZJ Gold, AJ Jensen, ED′ Agnese… - Ecology, 2023 - Wiley Online Library
Amplicon‐sequence data from environmental DNA (eDNA) and microbiome studies provide
important information for ecology, conservation, management, and health. At present …
important information for ecology, conservation, management, and health. At present …
DNA metabarcoding—Need for robust experimental designs to draw sound ecological conclusions
DNA metabarcoding, especially when coupled with high‐through‐put DNA sequencing, is
currently revolutionizing our capacity to assess biodiversity across a full range of taxa and …
currently revolutionizing our capacity to assess biodiversity across a full range of taxa and …
A pile of pipelines: An overview of the bioinformatics software for metabarcoding data analyses
A Hakimzadeh, A Abdala Asbun… - Molecular Ecology …, 2024 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding has gained growing attention as a strategy for
monitoring biodiversity in ecology. However, taxa identifications produced through …
monitoring biodiversity in ecology. However, taxa identifications produced through …
Minimizing polymerase biases in metabarcoding
DNA metabarcoding is an increasingly popular method to characterize and quantify
biodiversity in environmental samples. Metabarcoding approaches simultaneously amplify a …
biodiversity in environmental samples. Metabarcoding approaches simultaneously amplify a …
[HTML][HTML] SLIM: a flexible web application for the reproducible processing of environmental DNA metabarcoding data
Background High-throughput amplicon sequencing of environmental DNA (eDNA
metabarcoding) has become a routine tool for biodiversity survey and ecological studies. By …
metabarcoding) has become a routine tool for biodiversity survey and ecological studies. By …
[HTML][HTML] Understanding PCR processes to draw meaningful conclusions from environmental DNA studies
As environmental DNA (eDNA) studies have grown in popularity for use in ecological
applications, it has become clear that their results differ in significant ways from those of …
applications, it has become clear that their results differ in significant ways from those of …
Environmental DNA metabarcoding: Transforming how we survey animal and plant communities
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …
How quantitative is metabarcoding: A meta‐analytical approach
Metabarcoding has been used in a range of ecological applications such as taxonomic
assignment, dietary analysis and the analysis of environmental DNA. However, after a …
assignment, dietary analysis and the analysis of environmental DNA. However, after a …
Revisiting the effect of PCR replication and sequencing depth on biodiversity metrics in environmental DNA metabarcoding
Environmental DNA (eDNA) metabarcoding is an increasingly popular tool for measuring
and cataloguing biodiversity. Because the environments and substrates in which DNA is …
and cataloguing biodiversity. Because the environments and substrates in which DNA is …
Replication levels, false presences and the estimation of the presence/absence from eDNA metabarcoding data
GF Ficetola, J Pansu, A Bonin… - Molecular ecology …, 2015 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding is increasingly used to study the present and
past biodiversity. eDNA analyses often rely on amplification of very small quantities or …
past biodiversity. eDNA analyses often rely on amplification of very small quantities or …