Real‐time PCR

D Fraga, T Meulia, S Fenster - Current protocols essential …, 2008 - Wiley Online Library
Real‐time PCR is a recent modification to the polymerase chain reaction that allows precise
quantification of specific nucleic acids in a complex mixture by fluorescent detection of …

Real‐time PCR

D Fraga, T Meulia, S Fenster - Current protocols essential …, 2014 - Wiley Online Library
Real‐time PCR is a recent modification to the polymerase chain reaction that allows precise
quantification of specific nucleic acids in a complex mixture by fluorescent detection of …

Analysis and normalization of real-time polymerase chain reaction (PCR) experimental data

MR Green, J Sambrook - Cold Spring Harbor Protocols, 2018 - cshprotocols.cshlp.org
In real-time polymerase chain reaction (PCR), also called quantitative real-time PCR [or
simply quantitative PCR (qPCR)] or kinetic PCR, the amplification of DNA is monitored by …

[PDF][PDF] Real-time PCR

SA Bustin - Encyclopedia of diagnostic genomics and …, 2005 - gene-quantification.com
The real-time polymerase chain reaction (PCR) uses fluorescent reporter molecules to
monitor the production of amplification products during each cycle of the PCR reaction. This …

Developments in real-time PCR research and molecular diagnostics

SA Bustin - Expert review of molecular diagnostics, 2010 - Taylor & Francis
This meeting was designed to highlight the wide range of new methods, instruments and
applications that underlie the popularity of quantitative real-time PCR technology in all areas …

Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences

SA Bustin - Expert review of molecular diagnostics, 2005 - Taylor & Francis
Real-time fluorescence-based quantitative PCR has become established as the benchmark
technology for the quantification of nucleic acids, offering an immense choice of protocols …

Real-time PCR for mRNA quantitation

ML Wong, JF Medrano - Biotechniques, 2005 - Taylor & Francis
Real-time PCR has become one of the most widely used methods of gene quantitation
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …

Quantification using real-time PCR technology: applications and limitations

D Klein - Trends in molecular medicine, 2002 - cell.com
The introduction of real-time PCR technology has significantly improved and simplified the
quantification of nucleic acids, and this technology has become an invaluable tool for many …

High‐throughput real‐time quantitative reverse transcription PCR

AL Bookout, CL Cummins… - Current protocols in …, 2006 - Wiley Online Library
Extensive detail on the application of the real‐time quantitative polymerase chain reaction
(QPCR) for the analysis of gene expression is provided in this unit. The protocols are …

Prime time for real-time PCR

L Bonetta - Nature Methods, 2005 - nature.com
Prime time for real-time PCR | Nature Methods Skip to main content Thank you for visiting
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