Protein diffusion is crucial for understanding the formation of protein complexes in vivo and
has been the subject of many fluorescence microscopy studies in cells; however, such …

The cytoplasmic membrane forms the barrier between any cell's interior and the outside
world. It contains many proteins that enable essential processes such as the transmission of …

Visualizing and quantifying molecular motion and interactions inside living cells provides
crucial insight into the mechanisms underlying cell function. This has been achieved by …

Single‐molecule fluorescence super‐resolution imaging and tracking provide nanometer‐
scale information about subcellular protein positions and dynamics. These single‐molecule …

Fluorescence recovery after photobleaching and fluorescence correlation spectroscopy are
the primary means for studying translational diffusion in biological systems. Both techniques …

Lateral diffusion of proteins in the plane of a biological membrane is important for many vital
processes, including energy conversion, signaling, chemotaxis, cell division, protein …

By following single fluorescent molecules in a microscope, single-particle tracking (SPT) can
measure diffusion and binding on the nanometer and millisecond scales. Still, although SPT …

Since the advent of the green fluorescent protein, the subcellular localization, mobility,
transport routes and binding interactions of proteins can be studied in living cells. Live cell …

The trajectory of a single protein in the cytosol of a living cell contains information about its
molecular interactions in its native environment. However, it has remained challenging to …

Single-molecule imaging enables biophysical measurements devoid of ensemble
averaging, gives enhanced spatial resolution beyond the diffraction limit, and permits …