Metrics commonly used to describe antibiotic efficacy rely on measurements performed on
bacterial populations. However, certain cells in a bacterial population can continue to grow …

Mutations in the rifampicin (Rif)-binding site of RNA polymerase (RNAP) confer antibiotic
resistance and often have global effects on transcription that compromise fitness and stress …

Fitness costs play a key role in the evolutionary dynamics of antibiotic resistance in bacteria
by generating selection against resistance in the absence of antibiotics. Although the …

Bacteria can adapt to stressful conditions through mutations affecting the RNA polymerase
core subunits that lead to beneficial changes in transcription. In response to selection with …

The emergence of drug-resistant pathogens poses a major threat to public health. Although
influenced by multiple factors, high-level resistance is often associated with mutations in …

Antibiotic resistance often evolves by mutations at conserved sites in essential genes,
resulting in parallel molecular evolution between divergent bacterial strains and species …

In the last 40 years only one new antitubercular drug has been approved, whilst resistance
to current drugs, including rifampicin, is spreading. Here, we used the model organism …

Antibiotic treatment kills a large portion of a population, while a small, tolerant subpopulation
survives. Tolerant bacteria disrupt antibiotic efficacy and increase the likelihood that a …

The antibiotic trimethoprim (TMP) is used to treat a variety of Escherichia coli infections, but
its efficacy is limited by the rapid emergence of TMP-resistant bacteria. Previous laboratory …

Mycobacteria grow and divide asymmetrically, creating variability in growth pole age, growth
properties, and antibiotic susceptibilities. Here, we investigate the importance of growth pole …