BCR–ABL fusion proteins show increased signaling through their ABL tyrosine kinase
domain, which can be blocked by specific inhibitors, thereby providing effective treatment …

A rapid and simple polymerase chain reaction (PCR) method is described that is capable of
identifying any of the BCR-ABL transcripts that have yet been described in chronic myeloid …

Patients with chronic myeloid leukemia harbor the chromosomal translocation t (9; 22),
which corresponds to fusion of the BCR and ABL genes at the DNA level. The translated …

Tyrosine kinase inhibitors have profoundly modified the treatment and prognosis of chronic
myeloid leukemia and Ph+ acute lymphoblastic leukemia. A rapid and accurate detection of …

The number of genetic lesions necessary to generate leukemia in humans is unknown, but it
is possible that certain specific abnormalities, eg, fusion genes, known to be associated with …

Real-time RT-PCR has great advantages for estimating transcript levels in a variety of
situations. These include relative rapid assay times (hours), reliability and ease of …

The sensitivity and clinical utility of the polymerase chain reaction (PCR) assay for the
detection of BCR-ABL gene rearrangement was compared to conventional cytogenetics for …

Using fluorescence in situ hybridization (FISH), we were able to demonstrate 22-24-fold
amplification of the bcr/abl fusion gene in the human leukemic cell line K-562. About 60% of …

Chronic myelogenous leukemia is characterized by the presence of the reciprocal t (9;
22)(q34; q11) in which c-abl located on chromosome 9, and the bcr locus located on …

Quantifying bcr/abl fusion transcripts in chronic myelogenous leukemia is thought to serve
as a powerful parameter for monitoring the kinetic nature of this clonal disease in vivo and in …