Establishment of an efficient root mediated genetic transformation method for gene function verification in citrus

YX Xiao, M Dutt, H Ma, C Xiao, Z Tong, ZQ Wang… - Scientia …, 2023 - Elsevier
Although several methods of citrus genetic transformation have been studied and published,
it is still necessary to further improve the efficiency of genetic transformation and increase …

[HTML][HTML] A versatile Agrobacterium-based plant transformation system for genetic engineering of diverse citrus cultivars

MM Dominguez, CS Padilla, KK Mandadi - Frontiers in Plant Science, 2022 - frontiersin.org
Developing an efficient transformation system is vital in genetically engineering recalcitrant
crops, particularly trees. Here, we outline an Agrobacterium tumefaciens-based stable plant …

[HTML][HTML] Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation in citrus seeds and its application in gene functional analysis

M Wang, YY Qin, NN Wei, HY Xue… - Frontiers in Plant Science, 2023 - frontiersin.org
Highly efficient genetic transformation technology is beneficial for plant gene functional
research and molecular improvement breeding. However, the most commonly used …

Citrus biotechnology: Achievements, limitations and future directions

S Singh, MV Rajam - Physiology and Molecular Biology of Plants, 2009 - Springer
Citrus is one of the most important commercial and nutritional fruit crops in the world, hence
it needs to be improved to cater to the diverse needs of consumers and crop breeders …

An Improved Procedure for Agrobacterium-Mediated Transformation of 'Carrizo' Citrange

Y Li, D Tang, Z Liu, J Chen, B Cheng, R Kumar, H Yer… - Plants, 2022 - mdpi.com
Although several protocols for genetic transformation of citrus have been published, it is
highly desirable to further improve its efficiency. Here we report treatments of Agrobacterium …

[HTML][HTML] Genetic transformation in citrus

D Donmez, O Simsek, T Izgu, YA Kacar… - The Scientific World …, 2013 - ncbi.nlm.nih.gov
Citrus is one of the world's important fruit crops. Recently, citrus molecular genetics and
biotechnology work have been accelerated in the world. Genetic transformation, a …

[HTML][HTML] Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies

G Conti, B Xoconostle-Cázares… - Frontiers in Plant …, 2021 - frontiersin.org
Citrus are among the most prevailing fruit crops produced worldwide. The implementation of
effective and reliable breeding programs is essential for coping with the increasing demands …

Agrobacterium tumefaciens-mediated genetic transformation and plant regeneration from a complex tetraploid hybrid citrus rootstock

M Dutt, J Madhavaraj, JW Grosser - Scientia horticulturae, 2010 - Elsevier
Agrobacterium-mediated genetic transformation of a tetraploid “tetrazyg” citrus rootstock
selection 'Orange# 16'[Nova mandarin (Citrus reticulata Blanco)+ Hirado Buntan pummelo …

Highly efficient hairy root genetic transformation and applications in citrus

H Ma, X Meng, K Xu, M Li, FG Gmitter Jr, N Liu… - Frontiers in Plant …, 2022 - frontiersin.org
Highly efficient genetic transformation technology is greatly beneficial for crop gene function
analysis and precision breeding. However, the most commonly used genetic transformation …

Evaluation of parameters affecting Agrobacterium-mediated transformation of citrus

M Dutt, JW Grosser - Plant Cell, Tissue and Organ Culture (PCTOC), 2009 - Springer
An improved protocol for genetic transformation of juvenile explants of Carrizo (Citrus
sinensis Osb.× Poncirus trifoliata L. Raf.), Duncan (Citrus paradisi Macf.), Hamlin (Citrus …