Antibodies and related proteins comprise one of the largest and fastest-growing classes of
protein pharmaceuticals. A majority of such molecules are monoclonal antibodies; however …

In the past decade, remarkable advances in the production and use of antibodies as
therapeutic drugs and in research/diagnostic fields have led to their recognition as value …

Based on the recent development of NanoLuc luciferase (Nluc), a small (19 kDa), highly
stable, ATP independent, bioluminescent protein, an extremely robust and ultra high …

The actual biotechnology industry demands fast and economic upstream and downstream
processes to purify biomolecules. In this context, different purification techniques, that offer …

Half-antibody fragments are a promising reagent for biosensing, drug-delivery and labeling
applications, since exposure of the free thiol group in the Fc hinge region allows oriented …

Introduction Assessing the specificity of protein binders is an essential first step in protein
biomarker assay development. Affimers are novel protein binders and can potentially …

Gastric cancer (GC) remains a major cause of death worldwide mainly because of the late
detection in advanced stage. Recently, we proposed CD44v6 as a relevant marker for early …

This paper deals with the theoretical design and experimental validation of an affinity-based
continuous multi-column chromatography process for the purification of single-chain …

CD22 marker is a highly internalizing antigen which is located on the surface of B-cells and
is being used as a promising target for treatment of B cell malignancies. Monoclonal …

Antibody fragments (Fab) are often produced by recombinant methods in Escherichia coli as
no glycosylation is needed. Besides the correctly expressed Fab molecule, a multitude of …