Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

MP Mezzina, MT Manoli, MA Prieto… - Biotechnology …, 2021 - Wiley Online Library
Growing environmental concern sparks renewed interest in the sustainable production of
(bio) materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are …

Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

NL Bitzenhofer, L Kruse, S Thies… - Essays in …, 2021 - portlandpress.com
Biotechnological production in bacteria enables access to numerous valuable chemical
compounds. Nowadays, advanced molecular genetic toolsets, enzyme engineering as well …

(Chemo) biocatalytic upgrading of biobased furanic platforms to chemicals, fuels, and materials: a comprehensive review

N Li, MH Zong - ACS Catalysis, 2022 - ACS Publications
Biobased furans, such as furfural, 5-hydroxymethylfurfural, and 2, 5-furandicarboxylic acid,
are recognized as the top-value platform chemicals in the valorization of biomass to …

Modular (de) construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing

DC Volke, RA Martino, E Kozaeva, AM Smania… - Nature …, 2022 - nature.com
CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

PI Nikel, T Fuhrer, M Chavarría… - The ISME …, 2021 - academic.oup.com
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …

[HTML][HTML] Guiding stars to the field of dreams: Metabolically engineered pathways and microbial platforms for a sustainable lignin-based industry

F Weiland, M Kohlstedt, C Wittmann - Metabolic engineering, 2022 - Elsevier
Lignin is an important structural component of terrestrial plants and is readily generated
during biomass fractionation in lignocellulose processing facilities. Due to lacking …

[HTML][HTML] Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation

J Turlin, B Dronsella, A De Maria, SN Lindner… - Metabolic …, 2022 - Elsevier
Formate is a promising, water-soluble C1 feedstock for biotechnology that can be efficiently
produced from CO 2—but formatotrophy has been engineered in only a few industrially …

[HTML][HTML] A navigation guide of synthetic biology tools for Pseudomonas putida

M Martin-Pascual, C Batianis, L Bruinsma… - Biotechnology …, 2021 - Elsevier
Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …

Customized valorization of waste streams by Pseudomonas putida: State-of-the-art, challenges, and future trends

J Son, SH Lim, YJ Kim, HJ Lim, JY Lee, S Jeong… - Bioresource …, 2023 - Elsevier
Preventing catastrophic climate events warrants prompt action to delay global warming,
which threatens health and food security. In this context, waste management using …

Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes

N Gurdo, DC Volke, PI Nikel - Trends in Biotechnology, 2022 - cell.com
Major advances toward a bio-based industry enabled cost-efficient bioproduction of multiple
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …