The role of glycosylation in therapeutic antibodies

M Spearman, B Dionne, M Butler - Antibody expression and production, 2011 - Springer
Monoclonal antibodies (Mabs) are biopharmaceuticals that are used increasingly for the
treatment of a wide range of diseases such as cancer and autoimmunity. The effectiveness …

Characterization of Recombinant Thermococcus kodakaraensis (KOD) DNA Polymerases Produced Using Silkworm-Baculovirus Expression Vector System

M Yamashita, J Xu, D Morokuma, K Hirata… - Molecular …, 2017 - Springer
The KOD DNA polymerase from Thermococcus kodakarensis (Tkod-Pol) has been preferred
for PCR due to its rapid elongation rate, extreme thermostability and outstanding fidelity …

Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae

D Huang, YC Chao, Z Lv, JT Jan, YC Yang… - Journal of Veterinary …, 2021 - sciendo.com
Abstract Introduction: Novel clade 2.3. 4.4 H5 highly pathogenic avian influenza virus
(HPAIV) outbreaks have occurred since early 2015 in Taiwan and impacted the island …

Silkworm pupae function as efficient producers of recombinant glycoproteins with stable-isotope labeling

H Yagi, S Yanaka, R Yogo, A Ikeda, M Onitsuka… - Biomolecules, 2020 - mdpi.com
Baculovirus-infected silkworms are promising bioreactors for producing recombinant
glycoproteins, including antibodies. Previously, we developed a method for isotope labeling …

Efficient production of human Fas receptor extracellular domain–human IgG1 heavy chain Fc domain fusion protein using baculovirus/silkworm expression system

M Muraki, S Honda - Protein expression and purification, 2010 - Elsevier
The fusion protein consisting of human Fas receptor extracellular domain and human IgG1
heavy chain Fc domain (hFasRECD-Fc) is a medically important protein that potentially has …

Insect cell culture

MM van Oers, DE Lynn - eLS, 2010 - Wiley Online Library
Insect cell cultures are widely used in studies on insect cell physiology, developmental
biology and microbial pathology. In particular, insect cell culture is an indispensable tool for …

High yield exogenous protein HPL production in the Bombyx mori silk gland provides novel insight into recombinant expression systems

H Wang, L Wang, Y Wang, H Tao, W Yin, Y SiMa… - Scientific Reports, 2015 - nature.com
The silk gland of Bombyx mori (BmSG) has gained significant attention by dint of superior
synthesis and secretion of proteins. However, the application of BmSG bioreactor is still a …

Insect larvae: a new platform to produce commercial recombinant proteins

AM Targovnik, MB Arregui, LF Bracco… - Current …, 2016 - ingentaconnect.com
In Biotechnology, the expression of recombinant proteins is a constantly growing field and
different hosts are used for this purpose. Some valuable proteins cannot be produced using …

Human alpha 1-acid glycoprotein as a model protein for glycoanalysis in baculovirus expression vector system

D Morokuma, J Xu, H Mon, K Hirata, M Hino… - Journal of Asia-Pacific …, 2015 - Elsevier
Glycosylation is an important post-translational modification that confers various biological
activities, structural stability, and inter-molecular interactions to proteins. Baculovirus …

Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule

H Yagi, M Nakamura, J Yokoyama, Y Zhang… - Journal of biomolecular …, 2015 - Springer
Silkworms serve as promising bioreactors for the production of recombinant proteins,
including glycoproteins and membrane proteins, for structural and functional protein …