PTEN inhibitor and kit ligand increase in vitro activation and survival of primordial follicles in alpaca

G Rojas‐Guerra, D Dipaz‐Berrocal… - Anatomia, Histologia …, 2023 - Wiley Online Library
In mammals, activation of primordial follicles to primary follicle is a progressive and highly
regulated process. There is evidence in mice that phosphatase and tensin homologue …

Nerve growth factor promotes the survival of goat preantral follicles cultured in vitro

RN Chaves, A Alves, ABG Duarte, VR Araújo… - Cells Tissues …, 2010 - karger.com
The aim of this study was to investigate the effects of nerve growth factor (NGF) on the in
vitro culture of goat preantral follicles. Ovarian cortex fragments were cultured in α-MEM+ …

Alpha lipoic acid controls degeneration and ensures follicular development in ovine ovarian tissue cultured in vitro

LVS Ñaupas, FDR Gomes, ACA Ferreira, SM Morais… - Theriogenology, 2024 - Elsevier
This study aims to evaluate the effects of adding alpha lipoic acid (ALA) to the in vitro
ovarian tissue culture medium, either fresh or after vitrification/warming. For this purpose, 10 …

Protein Localization of Epidermal Growth Factor in Sheep Ovaries and Improvement of Follicle Survival and Antrum Formation In Vitro

LP Santos, VRP Barros, AYP Cavalcante… - … in Domestic Animals, 2014 - Wiley Online Library
Contents The aims of this study were to characterize EGF protein expression in ovine
ovaries and to verify the effect of EGF on the in vitro development of isolated pre‐antral …

Steady‐state level of kit ligand mRNA in goat ovaries and the role of kit ligand in preantral follicle survival and growth in vitro

JJH Celestino, JB Bruno, IB Lima‐Verde… - Molecular …, 2010 - Wiley Online Library
The aims of this study were to investigate steady‐state level of Kit Ligand (KL) mRNA and its
effects on in vitro survival and growth of caprine preantral follicles. RT‐PCR was used to …

Refining insulin concentrations in culture medium containing growth factors BMP15 and GDF9: An in vitro study of the effects on follicle development of goats

DJ Dipaz-Berrocal, NAR Sá, DD Guerreiro… - Animal reproduction …, 2017 - Elsevier
The aim of the present study was to investigate the influence of two insulin concentrations
(10 ng/mL and 10 μg/mL) combined or in the absence of BMP15 and/or GDF9, on the in vitro …

Heterotopic ovarian allotransplantation in a caprine model: effects of implant site on morphological parameters

ARS Vieira, LMCP Bersano, FAS Brandão… - Animal Reproduction …, 2024 - Elsevier
This study aimed to investigate a new implantation site (intra-auricular subcutaneous-IA)
compared to intramuscular (IM) in the cervical portion (cervical splenius muscle) of the neck …

Response of preantral follicles exposed to quinoxaline: A new compound with anticancer potential

DD Guerreiro, LF de Lima, NAR de Sá… - Research in veterinary …, 2020 - Elsevier
The culture of preantral follicles as an in vitro model to evaluate the toxicity of new
anticancer drug has being established. Therefore, the aim of this study was to evaluate the …

Formation and activation induction of primordial follicles using granulosa and cumulus cells conditioned media

MJ Atrabi, V Akbarinejad… - Journal of cellular …, 2019 - Wiley Online Library
Fertility preservation of prepubertal girls subjected to invasive cancer therapy necessitates
defining protocols for activation of isolated primordial follicles. Granulosa (GCs) and …

Recombinant epidermal growth factor maintains follicular ultrastructure and promotes the transition to primary follicles in caprine ovarian tissue cultured in vitro

JJH Celestino, JB Bruno, IB Lima-Verde… - Reproductive …, 2009 - Springer
We investigated the effects of epidermal growth factor on the survival and growth of caprine
preantral follicles. Ovarian fragments were cultured for 1 and 7 days in enriched minimal …