A new era in functional genomics screens
L Przybyla, LA Gilbert - Nature Reviews Genetics, 2022 - nature.com
The past 25 years of genomics research first revealed which genes are encoded by the
human genome and then a detailed catalogue of human genome variation associated with …
human genome and then a detailed catalogue of human genome variation associated with …
DNA repair pathway choices in CRISPR-Cas9-mediated genome editing
Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing
Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …
However, the currently most practicable approaches rely on the generation of DNA double …
[HTML][HTML] CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
[HTML][HTML] Mapping the genetic landscape of DNA double-strand break repair
Cells repair DNA double-strand breaks (DSBs) through a complex set of pathways critical for
maintaining genomic integrity. To systematically map these pathways, we developed a high …
maintaining genomic integrity. To systematically map these pathways, we developed a high …
[HTML][HTML] Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR …
RM Quadros, H Miura, DW Harms, H Akatsuka, T Sato… - Genome biology, 2017 - Springer
Background Conditional knockout mice and transgenic mice expressing recombinases,
reporters, and inducible transcriptional activators are key for many genetic studies and …
reporters, and inducible transcriptional activators are key for many genetic studies and …
[HTML][HTML] CRISPR-mediated base editing enables efficient disruption of eukaryotic genes through induction of STOP codons
Standard CRISPR-mediated gene disruption strategies rely on Cas9-induced DNA double-
strand breaks (DSBs). Here, we show that CRISPR-dependent base editing efficiently …
strand breaks (DSBs). Here, we show that CRISPR-dependent base editing efficiently …
[HTML][HTML] Kinetics and fidelity of the repair of Cas9-induced double-strand DNA breaks
EK Brinkman, T Chen, M de Haas, HA Holland… - Molecular cell, 2018 - cell.com
The RNA-guided DNA endonuclease Cas9 is a powerful tool for genome editing. Little is
known about the kinetics and fidelity of the double-strand break (DSB) repair process that …
known about the kinetics and fidelity of the double-strand break (DSB) repair process that …
[HTML][HTML] Methods and applications of CRISPR-mediated base editing in eukaryotic genomes
The past several years have seen an explosion in development of applications for the
CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to …
CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to …
[HTML][HTML] Cell cycle arrest and p53 prevent ON-target megabase-scale rearrangements induced by CRISPR-Cas9
G Cullot, J Boutin, S Fayet, F Prat, J Rosier… - Nature …, 2023 - nature.com
The CRISPR-Cas9 system has revolutionized our ability to precisely modify the genome and
has led to gene editing in clinical applications. Comprehensive analysis of gene editing …
has led to gene editing in clinical applications. Comprehensive analysis of gene editing …