Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

Formate is a promising, water-soluble C1 feedstock for biotechnology that can be efficiently
produced from CO 2—but formatotrophy has been engineered in only a few industrially …

Metabolic engineering holds the promise to transform the chemical industry and to support
the transition into a circular bioeconomy, by engineering cellular biocatalysts that efficiently …

Much of our current understanding of microbiology is based on the application of genetic
engineering procedures. Since their inception (more than 30 years ago), methods based …

The excessive consumption of fossil fuels causes massive emission of CO2, leading to
climate deterioration and environmental pollution. The development of substitutes and …

A lack of generic and effective genetic manipulation methods for Pseudomonas has
restricted fundamental research and utilization of this genus for biotechnology applications …

Owing to their physiological activities, plant-derived phenolic acids, such as protocatechuic
acid (PCA), have extensive applications and market prospects. However, traditional …

The presence of mineral fluoride (F−) in the environment has both a geogenic and
anthropogenic origin, and the halide has been described to be toxic in virtually all living …

Pseudomonas putida KT2440 is an attractive bacterial host for biotechnological production
of valuable chemicals from renewable lignocellulosic feedstocks as it can valorize lignin …