Role of the CrcB transporter of Pseudomonas putida in the multi‐level stress response elicited by mineral fluoride
The presence of mineral fluoride (F−) in the environment has both a geogenic and
anthropogenic origin, and the halide has been described to be toxic in virtually all living …
anthropogenic origin, and the halide has been described to be toxic in virtually all living …
[HTML][HTML] Challenges and opportunities in bringing nonbiological atoms to life with synthetic metabolism
R Haas, PI Nikel - Trends in Biotechnology, 2023 - cell.com
The relatively narrow spectrum of chemical elements within the microbial 'biochemical
palate'limits the reach of biotechnology, because several added-value compounds can only …
palate'limits the reach of biotechnology, because several added-value compounds can only …
Engineering of Pseudomonas putida for accelerated co-utilization of glucose and cellobiose yields aerobic overproduction of pyruvate explained by an upgraded …
Pseudomonas putida KT2440 is an attractive bacterial host for biotechnological production
of valuable chemicals from renewable lignocellulosic feedstocks as it can valorize lignin …
of valuable chemicals from renewable lignocellulosic feedstocks as it can valorize lignin …
Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and the Cascade–Cas3 system
W Zheng, Y Xia, X Wang, S Gao, D Zhou… - Nature …, 2023 - nature.com
A lack of generic and effective genetic manipulation methods for Pseudomonas has
restricted fundamental research and utilization of this genus for biotechnology applications …
restricted fundamental research and utilization of this genus for biotechnology applications …
Base editing for reprogramming cyanobacterium Synechococcus elongatus
SY Wang, X Li, SG Wang, PF Xia - Metabolic Engineering, 2023 - Elsevier
Cyanobacteria can directly convert carbon dioxide (CO 2) at the atmospheric level to
biofuels, value-added chemicals and food products, making them ideal candidates to …
biofuels, value-added chemicals and food products, making them ideal candidates to …
The pAblo· pCasso self-curing vector toolset for unconstrained cytidine and adenine base-editing in Gram-negative bacteria
E Kozaeva, ZS Nielsen… - Nucleic acids …, 2024 - academic.oup.com
A synthetic biology toolkit, exploiting clustered regularly interspaced short palindromic
repeats (CRISPR) and modified CRISPR-associated protein (Cas) base-editors, was …
repeats (CRISPR) and modified CRISPR-associated protein (Cas) base-editors, was …
High‐Complexity One‐Pot Golden Gate Assembly
Abstract Golden Gate Assembly is a flexible method of DNA assembly and cloning that
permits the joining of multiple fragments in a single reaction through predefined …
permits the joining of multiple fragments in a single reaction through predefined …
[HTML][HTML] Synthetic metabolism for in vitro acetone biosynthesis driven by ATP regeneration
E Kozaeva, M Nieto-Domínguez, AD Hernández… - RSC Chemical …, 2022 - pubs.rsc.org
In vitro ketone production continues to be a challenge due to the biochemical features of the
enzymes involved—even when some of them have been extensively characterized (eg …
enzymes involved—even when some of them have been extensively characterized (eg …
Recursive genome engineering decodes the evolutionary origin of an essential thymidylate kinase activity in Pseudomonas putida KT2440
Thymidylate kinases (TMPKs) play an essential role in DNA biosynthesis across all domains
of life by catalyzing dTMP phosphorylation to dTDP. In Pseudomonas putida KT2440, a …
of life by catalyzing dTMP phosphorylation to dTDP. In Pseudomonas putida KT2440, a …
[HTML][HTML] Revolutionizing biofuel generation: Unleashing the power of CRISPR-Cas mediated gene editing of extremophiles
Molecular biology techniques like gene editing have altered the specific genes in micro-
organisms to increase their efficiency to produce biofuels. This review paper investigates the …
organisms to increase their efficiency to produce biofuels. This review paper investigates the …