Tutorial: guidance for quantitative confocal microscopy
When used appropriately, a confocal fluorescence microscope is an excellent tool for
making quantitative measurements in cells and tissues. The confocal microscope's ability to …
making quantitative measurements in cells and tissues. The confocal microscope's ability to …
[HTML][HTML] Clarifying tissue clearing
DS Richardson, JW Lichtman - Cell, 2015 - cell.com
Biological specimens are intrinsically three dimensional; however, because of the obscuring
effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to …
effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to …
MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells
KC Gwosch, JK Pape, F Balzarotti, P Hoess… - Nature …, 2020 - nature.com
The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …
dimensional resolution at the size scale of a fluorescent marker. Here we show that by …
Orthogonally induced differentiation of stem cells for the programmatic patterning of vascularized organoids and bioprinted tissues
The generation of organoids and tissues with programmable cellular complexity,
architecture and function would benefit from the simultaneous differentiation of human …
architecture and function would benefit from the simultaneous differentiation of human …
Cell and nucleus shape as an indicator of tissue fluidity in carcinoma
Tissue, cell, and nucleus morphology change during tumor progression. In 2D confluent cell
cultures, different tissue states, such as fluid (unjammed) and solid (jammed), are correlated …
cultures, different tissue states, such as fluid (unjammed) and solid (jammed), are correlated …
Three-dimensional localization of single molecules for super-resolution imaging and single-particle tracking
Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …
two imaging modalities that illuminate the properties of cells and materials on spatial scales …
Actin cortex architecture regulates cell surface tension
Animal cell shape is largely determined by the cortex, a thin actin network underlying the
plasma membrane in which myosin-driven stresses generate contractile tension. Tension …
plasma membrane in which myosin-driven stresses generate contractile tension. Tension …
Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges
L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
into one of the most versatile and powerful imaging methods of the nanoscale over the past …
3D-printed microfluidic devices: fabrication, advantages and limitations—a mini review
C Chen, BT Mehl, AS Munshi, AD Townsend… - Analytical …, 2016 - pubs.rsc.org
A mini-review with 79 references. In this review, the most recent trends in 3D-printed
microfluidic devices are discussed. In addition, a focus is given to the fabrication aspects of …
microfluidic devices are discussed. In addition, a focus is given to the fabrication aspects of …
An introduction to optical super-resolution microscopy for the adventurous biologist
Ever since the inception of light microscopy, the laws of physics have seemingly thwarted
every attempt to visualize the processes of life at its most fundamental, sub-cellular, level …
every attempt to visualize the processes of life at its most fundamental, sub-cellular, level …