[HTML][HTML] Environmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods

J Pawlowski, K Bruce, K Panksep, FI Aguirre… - Science of the Total …, 2022 - Elsevier
Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for
identification of whole communities within a targeted group) is revolutionizing the field of …

[HTML][HTML] The future of biotic indices in the ecogenomic era: Integrating (e) DNA metabarcoding in biological assessment of aquatic ecosystems

J Pawlowski, M Kelly-Quinn, F Altermatt… - Science of the Total …, 2018 - Elsevier
The bioassessment of aquatic ecosystems is currently based on various biotic indices that
use the occurrence and/or abundance of selected taxonomic groups to define ecological …

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …

Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment

M Stat, MJ Huggett, R Bernasconi, JD DiBattista… - Scientific reports, 2017 - nature.com
Effective marine management requires comprehensive data on the status of marine
biodiversity. However, efficient methods that can document biodiversity in our oceans are …

Ecosystems monitoring powered by environmental genomics: a review of current strategies with an implementation roadmap

T Cordier, L Alonso‐Sáez… - Molecular …, 2021 - Wiley Online Library
A decade after environmental scientists integrated high‐throughput sequencing
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …

Assessing strengths and weaknesses of DNA metabarcoding‐based macroinvertebrate identification for routine stream monitoring

V Elbrecht, EE Vamos, K Meissner… - Methods in Ecology …, 2017 - Wiley Online Library
DNA metabarcoding holds great promise for the assessment of macroinvertebrates in
stream ecosystems. However, few large‐scale studies have compared the performance of …

Patterns of eukaryotic diversity from the surface to the deep-ocean sediment

T Cordier, IB Angeles, N Henry, F Lejzerowicz… - Science …, 2022 - science.org
Remote deep-ocean sediment (DOS) ecosystems are among the least explored biomes on
Earth. Genomic assessments of their biodiversity have failed to separate indigenous benthic …

DNA metabarcoding of littoral hard-bottom communities: high diversity and database gaps revealed by two molecular markers

OS Wangensteen, C Palacín, M Guardiola, X Turon - PeerJ, 2018 - peerj.com
Biodiversity assessment of marine hard-bottom communities is hindered by the high
diversity and size-ranges of the organisms present. We developed a DNA metabarcoding …

Population‐level inferences from environmental DNA—Current status and future perspectives

EE Sigsgaard, MR Jensen… - Evolutionary …, 2020 - Wiley Online Library
Environmental DNA (eDNA) extracted from water samples has recently shown potential as a
valuable source of population genetic information for aquatic macroorganisms. This …

Benchmarking DNA metabarcoding for biodiversity-based monitoring and assessment

E Aylagas, Á Borja, X Irigoien… - Frontiers in Marine …, 2016 - frontiersin.org
Characterization of biodiversity has been extensively used to confidently monitor and assess
environmental status. Yet, visual morphology, traditionally and widely used for species …