[HTML][HTML] Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing
CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …
decade and holds great promise for the treatment of human genetic diseases. Here we …
[HTML][HTML] CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
[HTML][HTML] Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites
CRISPR/Cas9-based genome editing has revolutionized experimental molecular biology
and entered the clinical world for targeted gene therapy. Identifying DNA modifications …
and entered the clinical world for targeted gene therapy. Identifying DNA modifications …
[PDF][PDF] The contribution of DNA repair pathways to genome editing and evolution in filamentous pathogens
DNA double-strand breaks require repair or risk corrupting the language of life. To ensure
genome integrity and viability, multiple DNA double-strand break repair pathways function in …
genome integrity and viability, multiple DNA double-strand break repair pathways function in …
Precise genome engineering in Drosophila using prime editing
JA Bosch, G Birchak… - Proceedings of the …, 2021 - National Acad Sciences
Precise genome editing is a valuable tool to study gene function in model organisms. Prime
editing, a precise editing system developed in mammalian cells, does not require double …
editing, a precise editing system developed in mammalian cells, does not require double …
[HTML][HTML] CRISPR/Cas9-mediated multi-allelic gene targeting in sugarcane confers herbicide tolerance
Sugarcane is the source of 80% of the sugar and 26% of the bioethanol produced globally.
However, its complex, highly polyploid genome (2 n= 100–120) impedes crop improvement …
However, its complex, highly polyploid genome (2 n= 100–120) impedes crop improvement …
Intratumoral heterogeneity and clonal evolution induced by HPV integration
The human papillomavirus (HPV) genome is integrated into host DNA in most HPV-positive
cancers, but the consequences for chromosomal integrity are unknown. Continuous long …
cancers, but the consequences for chromosomal integrity are unknown. Continuous long …
[HTML][HTML] CRISPR-Cas12a induced DNA double-strand breaks are repaired by multiple pathways with different mutation profiles in Magnaporthe oryzae
CRISPR-Cas mediated genome engineering has revolutionized functional genomics.
However, understanding of DNA repair following Cas-mediated DNA cleavage remains …
However, understanding of DNA repair following Cas-mediated DNA cleavage remains …
[HTML][HTML] Broadening the GMO risk assessment in the EU for genome editing technologies in agriculture
K Kawall, J Cotter, C Then - Environmental Sciences Europe, 2020 - Springer
Genome editing techniques, especially the CRISPR/Cas technology, increase the
possibilities and the speed of altering genetic material in organisms. So-called genome …
possibilities and the speed of altering genetic material in organisms. So-called genome …
Template plasmid integration in germline genome-edited cattle
AL Norris, SS Lee, KJ Greenlees, DA Tadesse… - Nature …, 2020 - nature.com
16526–16531 (2013). 9. Medugorac, I. et al. Bovine polledness—an autosomal dominant
trait with allelic heterogeneity. PLoS ONE 7, e39477 (2012). 10. Olsen, PA, Gelazauskaite …
trait with allelic heterogeneity. PLoS ONE 7, e39477 (2012). 10. Olsen, PA, Gelazauskaite …