Naked DNA has been shown to bind naturally to the sperm, a method called sperm-
mediated gene transfer (SMGT). Based on these observations, we examined the efficiency …

Attempts to use sperm cells for this purpose have been largely ineffective, probably due to
the need for efficient loading of DNA into the sperm cells. Liposomes were prepared by the …

We recently reported that exogenous DNA injected into testis as a liposome complex can be
transferred into the egg via sperm by natural mating and integrated in the genome (testis …

The efficacy of using liposomes to transfer DNA to chicken sperm cells was investigated.
Liposomes were prepared from dilauroyl (12: 0) phosphatidylcholine (DLPC), dimyristoyl …

Reasons for performing study: Sperm‐mediated gene transfer has been reported as a
method for production of transgenic animals in a variety of species, and this technique …

Liposomes were prepared in the presence of plasmid DNA and allowed to react with
spermatozoa from roosters prior to artificial insemination of 200 hens. Twelve days old …

In this study, we compared the transfection effectiveness of liposomes with the new
transfection reagent FuGene 6 in bovine sperm mediated gene transfer (SMGT) …

To investigate the potential use of sperm cells as vectors to transfer exogenous DNA via the
fertilization of oocytes into the germ line of mice, we have used liposomes to transfect DNA …

The production of transgenic animals by sperm cell-mediated gene transfer continues to be
a fascinating and intriguing possibility due to the simplicity and potential use of the …

Plasmid DNA was encapsulated in liposomes to protect from degradation and to support the
incorporation into sperm cells. Transgenic spermatozoa carry the foreign DNA to the target …